ICC (Immunocytochemistry)
(Immunocytochemistry/Immunofluorescence - Phospho-MCU(S92) antibody (14297) 14297 staining Phospho-MCU(S92) in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 14297 at a working dilution of 1/100. The secondary antibody was Alexa Fluor? 488 goat anti rabbit, used at a dilution of 1/500. The negative control is shown in bottom right hand panel - for the negative control, purified 14297 was used at a dilution of 1/100 followed by Alexa Fluor? 647 goat anti- rabbit antibody at a dilution of 1/500. Nuclei were counterstained with DAPI.)
Rabbit MCU Polyclonal Antibody | anti-MCU antibody
MCU (Phospho-Ser92) Rabbit pAb
ICC (Immunocytochemistry)
(Immunocytochemistry/Immunofluorescence - Phospho-MCU(S92) antibody (14297) 14297 staining Phospho-MCU(S92) in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 14297 at a working dilution of 1/100. The secondary antibody was Alexa Fluor? 488 goat anti rabbit, used at a dilution of 1/500. The negative control is shown in bottom right hand panel - for the negative control, purified 14297 was used at a dilution of 1/100 followed by Alexa Fluor? 647 goat anti- rabbit antibody at a dilution of 1/500. Nuclei were counterstained with DAPI.)
ICC (Immunocytochemistry)
(Immunocytochemistry/Immunofluorescence - Phospho-MCU(S92) antibody (14297) 14297 staining Phospho-MCU(S92) in the HeLa cell line by ICC/IF. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 14297 at a working dilution of 1/100. The secondary antibody was Alexa Fluor? 488 goat anti rabbit, used at a dilution of 1/500. The negative control is shown in bottom right hand panel - for the negative control, purified 14297 was used at a dilution of 1/100 followed by Alexa Fluor? 647 goat anti- rabbit antibody at a dilution of 1/500. Nuclei were counterstained with DAPI.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded rat lung tissue stained for Phospho-MCU(S92) using 14297 at 1/100 dilution in immunohistochemical analysis.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded rat lung tissue stained for Phospho-MCU(S92) using 14297 at 1/100 dilution in immunohistochemical analysis.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded mouse lung tissue stained for Phospho-MCU(S92) using 14297 at 1/100 dilution in immunohistochemical analysis.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded mouse lung tissue stained for Phospho-MCU(S92) using 14297 at 1/100 dilution in immunohistochemical analysis.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human lung carcinom tissue stained for Phospho-MCU(S92) using 14297 at 1/100 dilution in immunohistochemical analysis.)
IHC (Immunohistochemistry)
(Formalin-fixed, paraffin-embedded human lung carcinom tissue stained for Phospho-MCU(S92) using 14297 at 1/100 dilution in immunohistochemical analysis.)
WB (Western Blot)
(All lanes : Phospho-MCU(S92) Rabbit mAb at 1/1k dilutionLane 1 : 3T3 whole cell lysatesLane 2 : 293T whole cell lysatesLane 3 : 293T treated with Lambda Protein Phosphatase for 30min whole cell lysatesLane 4 : MCF7 whole cell lysatesLane 5 : MCF7 treated with Lambda Protein Phosphatase for 30min whole cell lysatesLysates/proteins at 20 ug per lane.Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 40 kDaObserved band size: 32 kDaExposure time: 10 seconds)
WB (Western Blot)
(All lanes : Phospho-MCU(S92) Rabbit mAb at 1/1k dilutionLane 1 : 3T3 whole cell lysatesLane 2 : 293T whole cell lysatesLane 3 : 293T treated with Lambda Protein Phosphatase for 30min whole cell lysatesLane 4 : MCF7 whole cell lysatesLane 5 : MCF7 treated with Lambda Protein Phosphatase for 30min whole cell lysatesLysates/proteins at 20 ug per lane.Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 40 kDaObserved band size: 32 kDaExposure time: 10 seconds)
WB (Western Blot)
(All lanes : Phospho-MCU(S92) Rabbit mAb at 1/1k dilutionLane 1 : MCF7 whole cell lysatesLane 2 : 293T whole cell lysatesLane 3 : A549 whole cell lysatesLane 4 : Rat Liver lysatesLysates/proteins at 20 ug per lane.,br>Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 40 kDaObserved band size: 32 kDaExposure time: 8 seconds)
WB (Western Blot)
(All lanes : Phospho-MCU(S92) Rabbit mAb at 1/1k dilutionLane 1 : MCF7 whole cell lysatesLane 2 : 293T whole cell lysatesLane 3 : A549 whole cell lysatesLane 4 : Rat Liver lysatesLysates/proteins at 20 ug per lane.,br>Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 40 kDaObserved band size: 32 kDaExposure time: 8 seconds)
IF (Immunofluorescence)
(Immunofluorescent analysis using the Antibody at 1:150 dilution.)
IF (Immunofluorescence)
(Immunofluorescent analysis using the Antibody at 1:150 dilution.)
Application Data
(All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)
Application Data
(All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)
Application Data
(All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)
Application Data
(All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)
Application Data
(All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)
Application Data
(All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)
Application Data
(All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)
Application Data
(All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)
NCBI and Uniprot Product Information
SDS-PAGE MW: 32 kDa
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Product Notes
The MCU mcu (Catalog #AAA314883) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MCU (Phospho-Ser92) Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MCU can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the MCU mcu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCU, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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