IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (MCU) Polyclonal Antibody, Unconjugated (bs-20189R) at 1:500 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)
Rabbit MCU Polyclonal Antibody | anti-MCU antibody
MCU Polyclonal Antibody
Gene Names
MCU; C10orf42; CCDC109A
Reactivity
Rat, Pig, Dog, Cow
Applications
Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MCU, Antibody; MCU Polyclonal Antibody; Calcium uniporter protein; mitochondrial; HsMCU; Coiled-coil domain-containing protein 109A; MCU; anti-MCU antibody
Host
Rabbit
Reactivity
Rat, Pig, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
Applicable Applications for anti-MCU antibody
IHC (Immunohistochemistry)
Function
Channel-forming and calcium-conducting subunit of the mitochondrial inner membrane calcium uniporter complex (uniplex) that mediates calcium uptake into the mitochondrial matrix (PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: , PubMed: ). MCU channel activity is regulated by MICU1 and MICU2 subunits of the uniplex. At low Ca(2+) levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca(2+) levels MICU1 increases MCU activity (PubMed: , PubMed: ). Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes (PubMed: ). While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells (PubMed: ). participates in mitochondrial permeability transition during ischemia-reperfusion injury (By similarity). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (PubMed: , PubMed: ). Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults (By similarity). Regulates synaptic vesicle endocytosis kinetics in central nerve terminal (By similarity). Involved in antigen processing and presentation (By similarity).
Cellular Location
Mitochondrion inner membrane; Multi-pass membrane protein
Preparation and Storage
Store at -20°C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4°C.
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (MCU) Polyclonal Antibody, Unconjugated (bs-20189R) at 1:500 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (MCU) Polyclonal Antibody, Unconjugated (bs-20189R) at 1:500 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39867
NCBI Official Full Name
calcium uniporter protein, mitochondrial isoform 2
NCBI Official Synonym Full Names
mitochondrial calcium uniporter
NCBI Official Symbol
MCU
NCBI Official Synonym Symbols
C10orf42; CCDC109A
NCBI Protein Information
calcium uniporter protein, mitochondrial; coiled-coil domain-containing protein 109A
UniProt Protein Name
Calcium uniporter protein, mitochondrial
UniProt Gene Name
MCU
UniProt Synonym Gene Names
C10orf42; CCDC109A
UniProt Entry Name
MCU_HUMAN
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Product Notes
The MCU mcu (Catalog #AAA291889) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MCU Polyclonal Antibody reacts with Rat, Pig, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's MCU can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the MCU mcu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCU, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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