Rabbit MDC1 Polyclonal Antibody | anti-MDC1 antibody

MDC1 Antibody

Cat. Num. AAA328898

• Gene Names: MDC1; NFBD1
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(88%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: MDC1, Antibody; MDC1 Antibody; Homologue to Drosophila photoreceptor protein calphotin; MDC 1; Mdc1; MDC1_HUMAN; Mediation of DNA damage checkpoint 1; Mediator of DNA damage checkpoint 1; Mediator of DNA damage checkpoint protein 1; NFBD 1; NFBD1; Nuclear factor with BRCT domains 1; Nuclear Factor with BRCT Domains Protein 1; anti-MDC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(88%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MDC1 Antibody detects endogenous levels of total MDC1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 2089

• Applicable Applications for anti-MDC1 antibody: ELISA
• Immunogen: A synthesized peptide derived from human MDC1, corresponding to a region within C-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohistochemisry)

(AAA328898 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohiostchemistry)

(AAA328898 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of extracts from HEK293, using MDC1 Antibody. The lane on the left was treated with blocking peptide.)

Related Product Information for anti-MDC1 antibody

Function: Required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. May serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AX. Also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis. ATM and CHEK2 may also be activated independently by a parallel pathway mediated by TP53BP1.

Post Translational Modifications: Phosphorylated upon exposure to ionizing radiation (IR), ultraviolet radiation (UV), and hydroxyurea (HU). Phosphorylation in response to IR requires ATM, NBN, and possibly CHEK2. Also phosphorylated during the G2/M phase of the cell cycle and during activation of the mitotic spindle checkpoint. Phosphorylation at Thr-4 by ATM stabilizes and enhances homodimerization via the FHA domain.Sumoylation at Lys-1840 by PIAS4 following DNA damage promotes ubiquitin-mediated degradation.Ubiquitinated by RNF4, leading to proteasomal degradation; undergoes 'Lys-48'-linked polyubiquitination.

Subcellular Location: Nucleus. Chromosome. Note: Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AX. Colocalizes with APTX at sites of DNA double-strand breaks.

Tissue Specificity: Highly expressed in testis.

Subunit Structure: Homodimer. Interacts with several proteins involved in the DNA damage response, although not all these interactions may be direct. Interacts with H2AX, which requires phosphorylation of H2AX on 'Ser-139'. Interacts with the MRN complex, composed of MRE11, RAD50, and NBN. Interacts with CHEK2, which requires ATM-mediated phosphorylation of 'Thr-68' within the FHA domain of CHEK2. Interacts constitutively with the BRCA1-BARD1 complex, SMC1A and TP53BP1. Interacts with ATM and FANCD2, and these interactions are reduced upon DNA damage. Also interacts with the PRKDC complex, composed of XRCC6/KU70, XRCC5/KU80 and PRKDC/XRCC7. This interaction may be required for PRKDC autophosphorylation, which is essential for DNA double strand break (DSB) repair. When phosphorylated by ATM, interacts with RNF8 (via FHA domain). Interacts with CEP164. When phosphorylated, interacts with APTX (via FHA-like domain).

Similarity: Tandemly repeated BRCT domains are characteristic of proteins involved in DNA damage signaling. In MDC1, these repeats are required for localization to chromatin which flanks sites of DNA damage marked by 'Ser-139' phosphorylation of H2AX.

NCBI and Uniprot Product Information

• NCBI GI #: 132626688
• NCBI GeneID: 9656
• NCBI Accession #: NP_055456.2
• NCBI GenBank Nucleotide #: NP_055456.2
• UniProt Accession #: Q14676
• Molecular Weight: Observed Molecular Weight: (Observed)226kD.; Predicted Molecular Weight: (Calculated)227kDa.
• NCBI Official Full Name: mediator of DNA damage checkpoint protein 1
• NCBI Official Synonym Full Names: mediator of DNA damage checkpoint 1
• NCBI Official Symbol: MDC1
• NCBI Official Synonym Symbols: NFBD1
• NCBI Protein Information: mediator of DNA damage checkpoint protein 1
• UniProt Protein Name: Mediator of DNA damage checkpoint protein 1
• UniProt Gene Name: MDC1
• UniProt Synonym Gene Names: KIAA0170; NFBD1
• UniProt Entry Name: MDC1_HUMAN