WB (Western Blot)
(Detection of human MED15 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MED15 antibody AAA211869 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. MED15 was also immunoprecipitated by rabbit anti-MED15 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
Rabbit anti-Human MED15 Polyclonal Antibody | anti-MED15 antibody
Rabbit anti-MED15 Antibody, Affinity Purified
Gene Names
MED15; TIG1; CAG7A; CTG7A; PCQAP; TIG-1; TNRC7; ARC105
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MED15, Antibody; Rabbit anti-MED15 Antibody, Affinity Purified; trinucleotide repeat-containing gene 7 protein; positive cofactor 2, glutamine/Q-rich-associated protein; TIG1; TIG-1; TNRC7; TPA inducible gene-1; TPA inducible protein; trinucleotide repeat containing 7; CTG7A; Positive cofactor 2 glutamine/Q-rich-associated protein; TPA-inducible gene 1 protein; CAG7A; PCQAP; activator-recruited cofactor 105 kDa component; mediator of RNA polymerase II transcription subunit 15; ARC105; CTG repeat protein 7a; Mediator complex subunit 15; PC2 (positive cofactor 2, multiprotein complex) glutamine/Q-rich-associated protein; PC2 glutamine/Q-rich-associated protein; PC2-glutamine-rich-associated protein; activator-recruited cofactor, 105-kD; Mediator of RNA polymerase II transcription subunit 15; mediator complex subunit 15; anti-MED15 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-MED15 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 475 and 525
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human MED15 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MED15 antibody AAA211869 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. MED15 was also immunoprecipitated by rabbit anti-MED15 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human MED15 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MED15 antibody AAA211869 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. MED15 was also immunoprecipitated by rabbit anti-MED15 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human MED15 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MED15 (Cat. No. AAA211869 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human MED15 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MED15 (Cat. No. AAA211869 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-MED15 antibody
Mediator complex subunit 15 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. MED15 is required for cholesterol-dependent gene regulation. It also positively regulates the Nodal signaling pathway. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96RN5].
Product Categories/Family for anti-MED15 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
86,753 Da
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 15 isoform a
NCBI Official Synonym Full Names
mediator complex subunit 15
NCBI Official Symbol
MED15
NCBI Official Synonym Symbols
TIG1; CAG7A; CTG7A; PCQAP; TIG-1; TNRC7; ARC105
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 15; TPA inducible gene-1; CTG repeat protein 7a; TPA inducible protein; TPA-inducible gene 1 protein; trinucleotide repeat containing 7; activator-recruited cofactor, 105-kD; PC2-glutamine-rich-associate
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 15
UniProt Gene Name
MED15
UniProt Synonym Gene Names
ARC105; CTG7A; PCQAP; TIG1; TNRC7; ARC105; PC2 glutamine/Q-rich-associated protein; TIG-1
UniProt Entry Name
MED15_HUMAN
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Product Notes
The MED15 med15 (Catalog #AAA211869) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MED15 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MED15 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MED15 med15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MED15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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