FCM/FACS (Flow Cytometry)
(MED7 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit MED7 Polyclonal Antibody | anti-MED7 antibody
MED7 Antibody (Center)
Gene Names
MED7; ARC34; CRSP9; CRSP33
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Pig)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MED7, Antibody; MED7 Antibody (Center); Mediator of RNA polymerase II transcription subunit 7; hMED7; Activator-recruited cofactor 34 kDa component; ARC34; Cofactor required for Sp1 transcriptional activation subunit 9; CRSP complex subunit 9; Mediator complex subunit 7; RNA polymerase transcriptional regulation mediator subunit 7 homolog; Transcriptional coactivator CRSP33; MED7; CRSP9; anti-MED7 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Pig)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MED7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-111 amino acids from the Central region of human MED7.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.28 (varies by lot)
Sequence Positions
83-111
Sequence Length
233
Applicable Applications for anti-MED7 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(MED7 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(MED7 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(MED7 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the MED7 antibody detected the MED7 protein (arrow).)
WB (Western Blot)
(MED7 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the MED7 antibody detected the MED7 protein (arrow).)
Related Product Information for anti-MED7 antibody
The activation of gene transcription is a multistep
process that is triggered by factors that recognize transcriptional
enhancer sites in DNA. These factors work with co-activators to
direct transcriptional initiation by the RNA polymerase II
apparatus. The protein encoded by this gene is a subunit of the
CRSP (cofactor required for SP1 activation) complex, which, along
with TFIID, is required for efficient activation by SP1. This
protein is also a component of other multisubunit complexes e.g.
thyroid hormone receptor-(TR-) associated proteins which interact
with TR and facilitate TR function on DNA templates in conjunction
with initiation factors and cofactors. Two transcript variants
encoding the same protein have been found for this gene. [provided
by RefSeq].
process that is triggered by factors that recognize transcriptional
enhancer sites in DNA. These factors work with co-activators to
direct transcriptional initiation by the RNA polymerase II
apparatus. The protein encoded by this gene is a subunit of the
CRSP (cofactor required for SP1 activation) complex, which, along
with TFIID, is required for efficient activation by SP1. This
protein is also a component of other multisubunit complexes e.g.
thyroid hormone receptor-(TR-) associated proteins which interact
with TR and facilitate TR function on DNA templates in conjunction
with initiation factors and cofactors. Two transcript variants
encoding the same protein have been found for this gene. [provided
by RefSeq].
Product Categories/Family for anti-MED7 antibody
References
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Sato, S., et al. J. Biol. Chem. 278(17):15123-15127(2003)
Ryu, S., et al. Proc. Natl. Acad. Sci. U.S.A. 96(13):7137-7142(1999)
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Sato, S., et al. J. Biol. Chem. 278(17):15123-15127(2003)
Ryu, S., et al. Proc. Natl. Acad. Sci. U.S.A. 96(13):7137-7142(1999)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27245
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 7
NCBI Official Synonym Full Names
mediator complex subunit 7
NCBI Official Symbol
MED7
NCBI Official Synonym Symbols
ARC34; CRSP9; CRSP33
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 7
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 7
UniProt Gene Name
MED7
UniProt Synonym Gene Names
ARC34; CRSP9; hMED7; ARC34; CRSP complex subunit 9
UniProt Entry Name
MED7_HUMAN
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Product Notes
The MED7 med7 (Catalog #AAA283797) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 83-111. The MED7 Antibody (Center) reacts with Human (Predicted Reactivity: Bovine, Mouse, Pig) and may cross-react with other species as described in the data sheet. AAA Biotech's MED7 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MED7 med7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MED7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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