WB (Western Blot)
(Anti-MAP2K3 rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:39 kDaObserved band size:39 kDa)
Rabbit MEK3/MKK3 Polyclonal Antibody | anti-MAP2K3 antibody
Anti-MEK3/MKK3 Antibody, Rabbit Polyclonal
Gene Names
MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
Reactivity
HumanPredicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Applications
Immunoprecipitation, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
MEK3/MKK3, Antibody; Anti-MEK3/MKK3 Antibody, Rabbit Polyclonal; MAP2K3/MKK3 Antibody, Rabbit PAb, Antigen Affinity Purified; mitogen-activated protein kinase kinase 3; Anti-MAPKK3 Antibody; Anti-MEK3 Antibody; Anti-MKK3 Antibody; Anti-PRKMK3 Antibody; Anti-SAPKK-2 Antibody; Anti-SAPKK2 Antibody; anti-MAP2K3 antibody
Host
Rabbit
Reactivity
Human
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human MEK3/MKK3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-MAP2K3 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human MAP2K3/MKK3
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human MAP2K3/MKK3, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-MAP2K3 rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:39 kDaObserved band size:39 kDa)
WB (Western Blot)
(Anti-MAP2K3 rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:39 kDaObserved band size:39 kDa)
IP (Immunoprecipitation)
(MAP2K3 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate1 uL anti-MAP2K3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-MAP2K3 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 39 kDaObserved band size: 39 kDa)
IP (Immunoprecipitation)
(MAP2K3 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate1 uL anti-MAP2K3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-MAP2K3 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 39 kDaObserved band size: 39 kDa)
Related Product Information for anti-MAP2K3 antibody
MAP2K3 (Mitogen-Activated Protein Kinase Kinase 3, also known as MKK3) is a Protein Coding gene. The protein encoded by this gene is a dual-specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress and participates in the MAP kinase-mediated signaling cascade. 3 alternatively spliced human isoforms have been reported. MAP2K3 is widely expressed in bone marrow, gall bladder, and other tissues. MAP2K3 resides in a biological pathway linked to memory. It is in a signaling cascade associated with beta-amyloid mediated apoptosis and has enriched expression in microglia.MAP2K3 may represent a novel therapeutic target for age-related memory decline and perhaps Alzheimer's disease (AD).
Product Categories/Family for anti-MAP2K3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,318 Da
NCBI Official Full Name
dual specificity mitogen-activated protein kinase kinase 3 isoform A
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase 3
NCBI Official Symbol
MAP2K3
NCBI Official Synonym Symbols
MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
NCBI Protein Information
dual specificity mitogen-activated protein kinase kinase 3; MEK 3; MAPKK 3; SAPK kinase 2; MAPK/ERK kinase 3; MAP kinase kinase 3; stress-activated protein kinase kinase 2
UniProt Protein Name
Dual specificity mitogen-activated protein kinase kinase 3
UniProt Gene Name
MAP2K3
UniProt Synonym Gene Names
MEK3; MKK3; PRKMK3; SKK2; MAP kinase kinase 3; MAPKK 3; MEK 3; SAPK kinase 2; SAPKK-2; SAPKK2
UniProt Entry Name
MP2K3_HUMAN
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Product Notes
The MAP2K3 map2k3 (Catalog #AAA254403) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MEK3/MKK3 Antibody, Rabbit Polyclonal reacts with Human Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology) and may cross-react with other species as described in the data sheet. AAA Biotech's MEK3/MKK3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MAP2K3 map2k3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEK3/MKK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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