IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K4 Antibody (S257) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit MEK4 (MAP2K4) Polyclonal Antibody | anti-MAP2K4 antibody
MEK4 (MAP2K4) Antibody (S257)
Gene Names
MAP2K4; JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1
Reactivity
Human (Predicted Reactivity: Xenopus, Mouse)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MEK4 (MAP2K4), Antibody; MEK4 (MAP2K4) Antibody (S257); Dual specificity mitogen-activated protein kinase kinase 4; MAP kinase kinase 4; MAPKK 4; JNK-activating kinase 1; MAPK/ERK kinase 4; MEK 4; SAPK/ERK kinase 1; SEK1; Stress-activated protein kinase kinase 1; SAPK kinase 1; SAPKK-1; SAPKK1; c-Jun N-terminal kinase kinase 1; JNKK; MAP2K4; JNKK1; MEK4; MKK4; PRKMK4; SERK1; SKK1; anti-MAP2K4 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Xenopus, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MEK4(MAP2K4) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 235-264 amino acids from human MEK4(MAP2K4).
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.35 (varies by lot)
Sequence Positions
235-264
Sequence Length
410
Applicable Applications for anti-MAP2K4 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K4 Antibody (S257) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with MAP2K4 Antibody (S257) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of MAP2K4 (arrow) using rabbit polyclonal MAP2K4 Antibody (S257). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K4 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of MAP2K4 (arrow) using rabbit polyclonal MAP2K4 Antibody (S257). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K4 gene (Lane 2) (Origene Technologies).)
Related Product Information for anti-MAP2K4 antibody
MAP2K4 is a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. MAP2K4 is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
Product Categories/Family for anti-MAP2K4 antibody
References
Robinson,V.L.,Mol. Cancer Res. 6 (3), 501-508 (2008)
Zhang,H.,J. Biol. Chem. 282 (20), 14788-14796 (2007)
Salmeron,A.,EMBO J. 15 (4), 817-826 (1996)
Zhang,H.,J. Biol. Chem. 282 (20), 14788-14796 (2007)
Salmeron,A.,EMBO J. 15 (4), 817-826 (1996)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44288
NCBI Official Full Name
dual specificity mitogen-activated protein kinase kinase 4 isoform 2
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase 4
NCBI Official Symbol
MAP2K4
NCBI Official Synonym Symbols
JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1
NCBI Protein Information
dual specificity mitogen-activated protein kinase kinase 4
UniProt Protein Name
Dual specificity mitogen-activated protein kinase kinase 4
UniProt Gene Name
MAP2K4
UniProt Synonym Gene Names
JNKK1; MEK4; MKK4; PRKMK4; SEK1; SERK1; SKK1; MAP kinase kinase 4; MAPKK 4; MEK 4; SEK1; SAPK kinase 1; SAPKK-1; SAPKK1; JNKK
UniProt Entry Name
MP2K4_HUMAN
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Product Notes
The MAP2K4 map2k4 (Catalog #AAA288840) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 235-264. The MEK4 (MAP2K4) Antibody (S257) reacts with Human (Predicted Reactivity: Xenopus, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's MEK4 (MAP2K4) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MAP2K4 map2k4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEK4 (MAP2K4), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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