WB (Western Blot)
(Western blot of Menin antibody. Nuclear extract of HEK293 cells (20 ?g) probed with Menin antibody (1:1,000).)
Rabbit anti-Human, Mouse Menin Polyclonal Antibody
Menin antibody (pAb)
Reactivity
Human, Mouse
Applications
Western Blot, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Affinity Purified
Synonyms
Menin, Antibody; Menin antibody (pAb); MEN1; MEAI; SCG2; anti-Menin antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-Menin antibody
WB (Western Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This Menin antibody was raised against a peptide corresponding to amino acids within the C-terminal region of human Menin.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(Western blot of Menin antibody. Nuclear extract of HEK293 cells (20 ?g) probed with Menin antibody (1:1,000).)
WB (Western Blot)
(Western blot of Menin antibody. Nuclear extract of HEK293 cells (20 ?g) probed with Menin antibody (1:1,000).)
ChIP (Chromatin Immunoprecipitation)
(Menin antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 25 ug of chromatin from a mouse thymus and 5 ug of MEN1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify MEN1 binding sites. MEN1 is a subunit of the MLL complex which catalyzes H3K4 trimethylation and is therefore expected to localize with H3K4me3. A sampling of the MEN1 ChIP-Seq data shows the expected co-localization of MEN1 and H3K4me3.)
ChIP (Chromatin Immunoprecipitation)
(Menin antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 25 ug of chromatin from a mouse thymus and 5 ug of MEN1 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15 million sequence tags were mapped to identify MEN1 binding sites. MEN1 is a subunit of the MLL complex which catalyzes H3K4 trimethylation and is therefore expected to localize with H3K4me3. A sampling of the MEN1 ChIP-Seq data shows the expected co-localization of MEN1 and H3K4me3.)
Related Product Information for anti-Menin antibody
Short Description: Our Menin antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq and Western blot; it has been shown to react with Human and Mouse samples.
Background: Menin (MEN1, SCG2) is a tumor suppressor and a component of multiple MLL-containing protein complexes (MLL, ACSOM, MLL2/3 and MLL3/4), which methylate histone H3 at lysine 4. Menin interacts directly with MLL through a domain in the N-terminus of MLL. It also interacts with RNA polymerase II large subunit C-terminal domain phosphorylated at Ser5. Menin is involved in the activation of HOX gene expression and is localized along with MLL at several HOX gene promoters. It is also important for activating the expression of several cell cycle regulators, indicating a mechanism for its role as a tumor suppressor. Defects in Menin are associated with inherited Multiple Endocrine Neoplasia type I, a dominant predisposition to cancer of a number of endocrine tissues.
Background: Menin (MEN1, SCG2) is a tumor suppressor and a component of multiple MLL-containing protein complexes (MLL, ACSOM, MLL2/3 and MLL3/4), which methylate histone H3 at lysine 4. Menin interacts directly with MLL through a domain in the N-terminus of MLL. It also interacts with RNA polymerase II large subunit C-terminal domain phosphorylated at Ser5. Menin is involved in the activation of HOX gene expression and is localized along with MLL at several HOX gene promoters. It is also important for activating the expression of several cell cycle regulators, indicating a mechanism for its role as a tumor suppressor. Defects in Menin are associated with inherited Multiple Endocrine Neoplasia type I, a dominant predisposition to cancer of a number of endocrine tissues.
Product Categories/Family for anti-Menin antibody
NCBI and Uniprot Product Information
Molecular Weight
80kDa
Similar Products
Product Notes
The Menin (Catalog #AAA59962) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Menin antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Menin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the Menin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Menin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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