WB (Western Blot)
(Anti- Menin Picoband antibody, AAA46398, Western blottingAll lanes: Anti Menin (AAA46398) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: 293T Whole Cell Lysate at 40ugLane 3: SMMC Whole Cell Lysate at 40ugLane 4: HEPA Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 68KDObserved bind size: 68KD)
anti-Human, Mouse Menin Polyclonal Antibody | anti-MEN1 antibody
Anti-Menin Antibody
Gene Names
MEN1; MEAI; SCG2
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Menin, Antibody; Anti-Menin Antibody; Menin; MEA 1; MEA1; MEN 1; MEN1; MEN1_HUMAN; Multiple Endocrine Adenomatosis 1; Multiple Endocrine Neoplasia 1; SCG 2; SCG2; Suppressor Candidate Gene 2; Wermer syndrome; ZES; Zollinger Ellison Syndrome; multiple endocrine neoplasia I; anti-MEN1 antibody
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
615
Applicable Applications for anti-MEN1 antibody
WB (Western Blot)
Immunogen
E Coli-derived human Menin recombinant protein (Position: P301-L615). Human Menin shares 93% and 94% amino acid (aa) sequence identity with mouse and rat Menin, respectively.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti- Menin Picoband antibody, AAA46398, Western blottingAll lanes: Anti Menin (AAA46398) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: 293T Whole Cell Lysate at 40ugLane 3: SMMC Whole Cell Lysate at 40ugLane 4: HEPA Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 68KDObserved bind size: 68KD)
WB (Western Blot)
(Anti- Menin Picoband antibody, AAA46398, Western blottingAll lanes: Anti Menin (AAA46398) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: 293T Whole Cell Lysate at 40ugLane 3: SMMC Whole Cell Lysate at 40ugLane 4: HEPA Whole Cell Lysate at 40ugLane 5: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 68KDObserved bind size: 68KD)
WB (Western Blot)
(Anti- Menin Picoband antibody, AAA46398, Western blottingAll lanes: Anti Menin (AAA46398) at 0.5ug/mlWB: Recombinant Human Menin Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
WB (Western Blot)
(Anti- Menin Picoband antibody, AAA46398, Western blottingAll lanes: Anti Menin (AAA46398) at 0.5ug/mlWB: Recombinant Human Menin Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
Related Product Information for anti-MEN1 antibody
Description: Rabbit IgG polyclonal antibody for Menin(MEN1) detection. Tested with WB in Human;Mouse.
Background: The MEN1 gene encodes menin, a nuclear scaffold protein that regulates gene transcription by coordinating chromatin remodeling. It is mapped to 11q13.1. MEN1 is considered to act as a tumor suppressor gene. It has been found that that MEN1 inactivation by antisense RNA antagonizes transforming growth factor-beta-mediated cell growth inhibition. Overexpression of MEN1 in an inducible cell culture system downregulated the proximal promoter. In vitro studies have shown that MEN1 is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD. What's more, MEN1 was essential to maintain MLL-associated myeloid transformation.
Background: The MEN1 gene encodes menin, a nuclear scaffold protein that regulates gene transcription by coordinating chromatin remodeling. It is mapped to 11q13.1. MEN1 is considered to act as a tumor suppressor gene. It has been found that that MEN1 inactivation by antisense RNA antagonizes transforming growth factor-beta-mediated cell growth inhibition. Overexpression of MEN1 in an inducible cell culture system downregulated the proximal promoter. In vitro studies have shown that MEN1 is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD. What's more, MEN1 was essential to maintain MLL-associated myeloid transformation.
References
1. Canaff, L., Vanbellinghen, J.-F., Kaji, H., Goltzman, D., Hendy, G. N. Impaired transforming growth factor-beta (TGF-beta) transcriptional activity and cell proliferation control of a menin in-frame deletion mutant associated with multiple endocrine neoplasia type 1 (MEN1). J. Biol. Chem. 287: 8584-8597, 2012. 2. Kaji, H., Canaff, L., Lebrun, J.-J., Goltzman, D., Hendy, G. N. Inactivation of menin, a Smad3-interacting protein, blocks transforming growth factor type beta signaling. Proc. Nat. Acad. Sci. 98: 3837-3842, 2001. 3. Zablewska, B., Bylund, L., Mandic, S. A., Fromaget, M., Gaudray, P., Weber, G. Transcription regulation of the multiple endocrine neoplasia type 1 gene in human and mouse. J. Clin. Endocr. Metab. 88: 3845-3851, 2003.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
63,748 Da
NCBI Official Full Name
menin isoform 1
NCBI Official Synonym Full Names
menin 1
NCBI Official Symbol
MEN1
NCBI Official Synonym Symbols
MEAI; SCG2
NCBI Protein Information
menin
UniProt Protein Name
Menin
UniProt Gene Name
MEN1
UniProt Synonym Gene Names
SCG2
UniProt Entry Name
MEN1_HUMAN
Similar Products
Product Notes
The MEN1 men1 (Catalog #AAA46398) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Menin Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Menin can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the MEN1 men1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Menin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.