WB (Western Blot)
(Detection of human and mouse MEPCE by western blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-MEPCE antibody AAA212536 (lot AAA212536-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human, Mouse MEPCE Polyclonal Antibody | anti-MEPCE antibody
Rabbit anti-MEPCE Antibody, Affinity Purified
Gene Names
MEPCE; BCDIN3
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MEPCE, Antibody; Rabbit anti-MEPCE Antibody, Affinity Purified; bin3 homolog; bin3, bicoid-interacting 3, homolog; BCDIN3; 7SK snRNA methylphosphate capping enzyme; MePCE; bicoid-interacting protein 3 homolog; methylphosphate capping enzyme; anti-MEPCE antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MEPCE antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 200 and 250
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse MEPCE by western blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-MEPCE antibody AAA212536 (lot AAA212536-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human and mouse MEPCE by western blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-MEPCE antibody AAA212536 (lot AAA212536-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human MEPCE by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-MEPCE antibody AAA212536 (lot AAA212536-1) used for IP at 6 ug/mg lysate. MEPCE was also immunoprecipitated by rabbit anti-MEPCE antibody For blotting immunoprecipitated MEPCE, AAA212536 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human MEPCE by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-MEPCE antibody AAA212536 (lot AAA212536-1) used for IP at 6 ug/mg lysate. MEPCE was also immunoprecipitated by rabbit anti-MEPCE antibody For blotting immunoprecipitated MEPCE, AAA212536 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human MEPCE by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MEPCE (Cat. No. AAA212536 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human MEPCE by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MEPCE (Cat. No. AAA212536 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-MEPCE antibody
7SK snRNA methylphosphate capping enzyme (MEPC) is an S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q7L2J0].
Product Categories/Family for anti-MEPCE antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,950 Da
NCBI Official Full Name
7SK snRNA methylphosphate capping enzyme isoform A
NCBI Official Synonym Full Names
methylphosphate capping enzyme
NCBI Official Symbol
MEPCE
NCBI Official Synonym Symbols
BCDIN3
NCBI Protein Information
7SK snRNA methylphosphate capping enzyme; bin3 homolog; bin3, bicoid-interacting 3, homolog; bicoid-interacting protein 3 homolog
UniProt Protein Name
7SK snRNA methylphosphate capping enzyme
UniProt Gene Name
MEPCE
UniProt Synonym Gene Names
BCDIN3; MePCE; Bin3 homolog
UniProt Entry Name
MEPCE_HUMAN
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Product Notes
The MEPCE mepce (Catalog #AAA212536) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MEPCE Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MEPCE can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MEPCE mepce for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEPCE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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