IP (Immunoprecipitation)
(Detection of human MERIT40 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MERIT40 antibody AAA211907 (lot AAA211907-2) used for IP at 6 ug per reaction. MERIT40 was also immunoprecipitated by a previous lot of this antibody (AAA211907-1) and rabbit anti-MERIT40 antibody For blotting immunoprecipitated MERIT40, AAA211907 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human MERIT40 Polyclonal Antibody | anti-MERIT40 antibody
Rabbit anti-MERIT40 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
MERIT40, Antibody; Rabbit anti-MERIT40 Antibody, Affinity Purified; C19orf62; new component of the BRCAA1 A complex; new component of the BRCA1-A complex; NBA1; MERIT40; mediator of RAP80 interactions and targeting subunit of 40 kDa; HSPC142; BRISC and BRCA1-A complex member 1; BRCA1-A complex subunit MERIT40; mediator of Rap80 interactions and targeting 40 kDa; BRISC and BRCA1 A complex member 1; anti-MERIT40 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MERIT40 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Mouse, Rat, Bovine, Orangutan
Immunogen
Between 175 and 225
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human MERIT40 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MERIT40 antibody AAA211907 (lot AAA211907-2) used for IP at 6 ug per reaction. MERIT40 was also immunoprecipitated by a previous lot of this antibody (AAA211907-1) and rabbit anti-MERIT40 antibody For blotting immunoprecipitated MERIT40, AAA211907 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human MERIT40 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MERIT40 antibody AAA211907 (lot AAA211907-2) used for IP at 6 ug per reaction. MERIT40 was also immunoprecipitated by a previous lot of this antibody (AAA211907-1) and rabbit anti-MERIT40 antibody For blotting immunoprecipitated MERIT40, AAA211907 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human MERIT40 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MERIT40 (Cat. No. AAA211907 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human MERIT40 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MERIT40 (Cat. No. AAA211907 Lot2) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-MERIT40 antibody
MERIT40 (mediator of RAP80 interactions and targeting subunit of 40 kDa) is a component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites and leads to targeting of the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. MERIT40 probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9NWV8].
Product Categories/Family for anti-MERIT40 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The MERIT40 babam1 (Catalog #AAA211907) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MERIT40 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MERIT40 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the MERIT40 babam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MERIT40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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