WB (Western Blot)
(Western blot analysis of extracts from A-431 , using Phospho-MERIT40 (Ser29) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
Rabbit MERIT40 Polyclonal Antibody | anti-MERIT40 antibody
Phospho-MERIT40 (Ser29) Antibody
Gene Names
BABAM1; NBA1; HSPC142; MERIT40; C19orf62
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Xenopus (92%)
Applications
ELISA, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
MERIT40, Antibody; Phospho-MERIT40 (Ser29) Antibody; BABA1_HUMAN; babam1; BRCA1 A complex subunit MERIT40; BRISC and BRCA1-A complex member 1; C19orf62; FLJ20571; HSPC142; hypothetical protein LOC29086; Mediator of Rap80 interactions and targeting 40kDa; Mediator of RAP80 interactions and targeting subunit of 40kDa; MERIT 40; MERIT40; NBA1; New component of the BRCA1 A complex; New component of the BRCA1-A complex; Uncharacterized protein C19orf62; anti-MERIT40 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Xenopus (92%)
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Xenopus (92%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-MERIT40 (Ser29) Antibody detects endogenous levels of MERIT40 only when phosphorylated at Ser29.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-MERIT40 antibody
ELISA (Peptide ELISA), WB (Western Blot)
Immunogen
A synthesized peptide derived from human MERIT40 around the phosphorylation site of Ser29.
Conjugation
Unconjugated
Fragment
Fab fragment
Subunit Structure
Component of the ARISC complex, at least composed of UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Component of the BRCA1-A complex, at least composed of BRCA1, BARD1, UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with ABRAXAS1 and BABAM2. Component of the BRISC complex, at least composed of ABRAXAS2, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Identified in a complex with SHMT2 and the other subunits of the BRISC complex.
Similarity
The VWFA-like region is similar to the VWFA domain. Its presence reveals similarities between the structure of the 19S proteasome and the BRCA1-A complexes.Belongs to the BABAM1 family.
Subcellular Location
Cytoplasm. Nucleus.
Note: Localizes at sites of DNA damage at double-strand breaks (DSBs).
Note: Localizes at sites of DNA damage at double-strand breaks (DSBs).
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from A-431 , using Phospho-MERIT40 (Ser29) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from A-431 , using Phospho-MERIT40 (Ser29) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells(LPS 4h treatment), using Phospho-MERIT40 (Ser29) Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells(LPS 4h treatment), using Phospho-MERIT40 (Ser29) Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-MERIT40 antibody
Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin in various substrates. In these 2 complexes, it is probably required to maintain the stability of BABAM2 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36 component. The BRISC complex is required for normal mitotic spindle assembly and microtubule attachment to kinetochores via its role in deubiquitinating NUMA1. Plays a role in interferon signaling via its role in the deubiquitination of the interferon receptor IFNAR1; deubiquitination increases IFNAR1 activity by enhancing its stability and cell surface expression. Down-regulates the response to bacterial lipopolysaccharide (LPS) via its role in IFNAR1 deubiquitination.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18,621 Da
NCBI Official Full Name
BRISC and BRCA1-A complex member 1 isoform 1
NCBI Official Synonym Full Names
BRISC and BRCA1 A complex member 1
NCBI Official Symbol
BABAM1
NCBI Official Synonym Symbols
NBA1; HSPC142; MERIT40; C19orf62
NCBI Protein Information
BRISC and BRCA1-A complex member 1
UniProt Protein Name
BRISC and BRCA1-A complex member 1
UniProt Gene Name
BABAM1
UniProt Entry Name
BABA1_HUMAN
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Product Notes
The MERIT40 babam1 (Catalog #AAA329492) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-MERIT40 (Ser29) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Xenopus (92%) and may cross-react with other species as described in the data sheet. AAA Biotech's MERIT40 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), WB (Western Blot). Researchers should empirically determine the suitability of the MERIT40 babam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MERIT40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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