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IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min))
Rabbit MET Polyclonal Antibody | anti-MET antibody
MET Antibody
Gene Names
MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antigen Affinity Purification
Synonyms
MET, Antibody; MET Antibody; HGFR; AUTS9; RCCP2; c-Met; anti-MET antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total MET protein.
Purity/Purification
Antigen Affinity Purification
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide
Concentration
1.8 mg/ml (varies by lot)
Sequence Length
1390
Applicable Applications for anti-MET antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
IHC: 100-300
WB 1:500 - 1:2000
IF 1:200 - 1:1000
WB 1:500 - 1:2000
IF 1:200 - 1:1000
Immunogen
Synthetic peptide of human MET
Preparation and Storage
Store at -20°C
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min))
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min))
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide)
WB (Western Blot)
(Western blot analysis of lysates from HepG2 cells. The lane on the right is blocked with the synthesized peptide.)
WB (Western Blot)
(Western blot analysis of lysates from HepG2 cells. The lane on the right is blocked with the synthesized peptide.)
WB (Western Blot)
(Western Blot analysis of various cells)
WB (Western Blot)
(Western Blot analysis of various cells)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/30,)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human esophagus cancer tissue using at dilution 1/30,)
WB (Western Blot)
(Gel: 6%SDS-PAGE Lysate: 40 μ g Lane: HepG2 cells Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 minutes)
WB (Western Blot)
(Gel: 6%SDS-PAGE Lysate: 40 μ g Lane: HepG2 cells Primary antibody: 1/200 dilution Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 minutes)
Related Product Information for anti-MET antibody
This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers. [provided by RefSeq, May 2016],
Product Categories/Family for anti-MET antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 145kD
NCBI Official Full Name
hepatocyte growth factor receptor isoform b
NCBI Official Synonym Full Names
MET proto-oncogene, receptor tyrosine kinase
NCBI Official Symbol
MET
NCBI Official Synonym Symbols
HGFR; AUTS9; RCCP2; c-Met; DFNB97
NCBI Protein Information
hepatocyte growth factor receptor
UniProt Protein Name
Hepatocyte growth factor receptor
UniProt Gene Name
MET
UniProt Synonym Gene Names
HGF receptor; SF receptor
UniProt Entry Name
MET_HUMAN
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Product Notes
The MET met (Catalog #AAA29724) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MET Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MET can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). IHC: 100-300 WB 1:500 - 1:2000 IF 1:200 - 1:1000. Researchers should empirically determine the suitability of the MET met for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MET, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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