Rabbit anti-Human Metabotropic Glutamate Receptor 6 (GPRC1F) Polyclonal Antibody | anti-GRM6 antibody

Metabotropic Glutamate Receptor 6 (GPRC1F) Antibody (C-term)

Cat. Num. AAA287267

• Gene Names: GRM6; mGlu6; CSNB1B; GPRC1F; MGLUR6
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Metabotropic Glutamate Receptor 6 (GPRC1F), Antibody; Metabotropic Glutamate Receptor 6 (GPRC1F) Antibody (C-term); Metabotropic glutamate receptor 6; mGluR6; GRM6; GPRC1F; MGLUR6; anti-GRM6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This Metabotropic Glutamate Receptor 6 (GPRC1F) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 832-861 amino acids from the C-terminal region of human Metabotropic Glutamate Receptor 6 (GPRC1F).
• Purity/Purification: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 832-861
• Sequence Length: 877

• Applicable Applications for anti-GRM6 antibody: FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
• Function: G-protein coupled receptor for glucamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase activity (by similarity). Signaling stimulates TRPM1 channel activity and Ca(2+) uptake. Required for normal vision.
• Cellular Location: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cell projection, dendrite. Note: Subject to trafficking from the endoplasmic reticulum to the Golgi apparatus and then to the cell membrane
• Tissue Location: Detected in melanocytes
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

FCM/FACS (Flow Cytometry)

(Metabotropic Glutamate Receptor 6 (GPRC1F) Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(GPRC1F Antibody (P847) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GPRC1F antibody detected the GPRC1F protein (arrow).)

Related Product Information for anti-GRM6 antibody

L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities.

Product Categories/Family for anti-GRM6 antibody

Neuroscience; Signal Transduction; GPCR Antibodies

References

Hashimoto, T., et al., Eur. J. Neurosci. 9(6):1226-1235 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 110611176
• NCBI GeneID: 2916
• NCBI Accession #: NP_000834.2
• NCBI GenBank Nucleotide #: NM_000843.3
• UniProt Accession #: O15303
• Molecular Weight: Calculated: 95468
• NCBI Official Full Name: metabotropic glutamate receptor 6
• NCBI Official Synonym Full Names: glutamate receptor, metabotropic 6
• NCBI Official Symbol: GRM6
• NCBI Official Synonym Symbols: mGlu6; CSNB1B; GPRC1F; MGLUR6
• NCBI Protein Information: metabotropic glutamate receptor 6
• UniProt Protein Name: Metabotropic glutamate receptor 6
• UniProt Gene Name: GRM6
• UniProt Synonym Gene Names: GPRC1F; MGLUR6; mGluR6
• UniProt Entry Name: GRM6_HUMAN