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product-image-AAA225127_IHC11.jpg IHC (Immunohistochemisry) (Immunohistochemical staining of paraffin-embedded Testis using MIA3 antibody)

Rabbit MIA3 Polyclonal Antibody | anti-MIA3 antibody

MIA3 Antibody

Gene Names
MIA3; ARNT; D320; TANGO; TANGO1; UNQ6077
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Antigen-Affinity Chromatography
Synonyms
MIA3, Antibody; MIA3 Antibody; Affinity Purified Rabbit Polyclonal MIA3 Antibody; Polyclonal MIA3 antibody; Anti-MIA3 antibody; MIA3 antibody; ARNT antibody; MIA-3; FLJ39207 antibody; D320 antibody; melanoma inhibitory activity family member 3 antibody; MIA-3 antibody; TANGO antibody; UNQ6077 antibody; KIAA0268 antibody; TANGO1 antibody; MIA 3 antibody; transport and Golgi organization protein 1 antibody; MIA 3; C219 reactive peptide antibody; melanoma inhibitory activity protein 3 antibody; C219-reactive peptide antibody; MIA3; FLJ21469 antibody; anti-MIA3 antibody
Ordering
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 1X PBS, 40% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
467
Applicable Applications for anti-MIA3 antibody
WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
MIA3 antibody was raised in rabbit using a recombinant fragment corresponding to a region within amino acids 1 and 150 of MIA3
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

IHC (Immunohistochemisry)

(Immunohistochemical staining of paraffin-embedded Testis using MIA3 antibody)

product-image-AAA225127_IHC11.jpg IHC (Immunohistochemisry) (Immunohistochemical staining of paraffin-embedded Testis using MIA3 antibody)

WB (Western Blot)

(Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 7.5% SDS PAGE gel and MIA3 antibody at a dilution of 1:1000)

product-image-AAA225127_WB13.jpg WB (Western Blot) (Western blot analysis of 30 ug of whole cell lysate (A: Hela) using a 7.5% SDS PAGE gel and MIA3 antibody at a dilution of 1:1000)

IF (Immunofluorescence)

(Sample: HepG2 cells were fixed in - 20 deg C 100% MeOH for 5 min. Green: MIA3 protein stained by MIA3 antibody diluted at 1:500. Blue: Hoechst 33343 staining.  )

product-image-AAA225127_IF15.jpg IF (Immunofluorescence) (Sample: HepG2 cells were fixed in - 20 deg C 100% MeOH for 5 min. Green: MIA3 protein stained by MIA3 antibody diluted at 1:500. Blue: Hoechst 33343 staining.  )
Related Product Information for anti-MIA3 antibody
Required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. May participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1.
Product Categories/Family for anti-MIA3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
214 kDa
NCBI Official Full Name
MIA3 protein, partial
NCBI Official Synonym Full Names
MIA SH3 domain ER export factor 3
NCBI Official Symbol
MIA3
NCBI Official Synonym Symbols
ARNT; D320; TANGO; TANGO1; UNQ6077
NCBI Protein Information
transport and Golgi organization protein 1 homolog

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Product Notes

The MIA3 (Catalog #AAA225127) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MIA3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the MIA3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIA3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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