WB (Western Blot)
(Detection of human MIA3/TANGO by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIA3/TANGO antibody AAA212980 (lot AAA212980-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Rabbit anti-Human MIA3/TANGO Polyclonal Antibody | anti-MIA3/TANGO antibody
Rabbit anti-MIA3/TANGO Antibody, Affinity Purified
Gene Names
MIA3; ARNT; D320; TANGO; TANGO1; UNQ6077
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MIA3/TANGO, Antibody; Rabbit anti-MIA3/TANGO Antibody, Affinity Purified; C219-reactive peptide; transport and Golgi organization protein 1 homolog; transport and Golgi organization protein 1; TANGO1; TANGO; MIA family member 3, ER export factor; melanoma inhibitory activity protein 3; D320; UNQ6077; ARNT; melanoma inhibitory activity family, member 3; Melanoma inhibitory activity protein 3; MIA SH3 domain ER export factor 3; anti-MIA3/TANGO antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MIA3/TANGO antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human MIA3/TANGO by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIA3/TANGO antibody AAA212980 (lot AAA212980-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of human MIA3/TANGO by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIA3/TANGO antibody AAA212980 (lot AAA212980-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human MIA3/TANGO by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIA3/TANGO antibody AAA212980 (lot AAA212980-1) used for IP at 6 ug per reaction. MIA3/TANGO was also immunoprecipitated by rabbit anti-MIA3/TANGO antibody BL18939. For blotting immunoprecipitated MIA3/TANGO, AAA212980 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human MIA3/TANGO by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MIA3/TANGO antibody AAA212980 (lot AAA212980-1) used for IP at 6 ug per reaction. MIA3/TANGO was also immunoprecipitated by rabbit anti-MIA3/TANGO antibody BL18939. For blotting immunoprecipitated MIA3/TANGO, AAA212980 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-MIA3/TANGO antibody
Melanoma inhibitory activity protein 3 (MIA3) is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q5JRA6].
Product Categories/Family for anti-MIA3/TANGO antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
213,702 Da
NCBI Official Full Name
melanoma inhibitory activity protein 3 isoform 1
NCBI Official Synonym Full Names
melanoma inhibitory activity family, member 3
NCBI Official Symbol
MIA3
NCBI Official Synonym Symbols
ARNT; D320; TANGO; TANGO1; UNQ6077
NCBI Protein Information
melanoma inhibitory activity protein 3; C219-reactive peptide; transport and Golgi organization protein 1
UniProt Protein Name
Melanoma inhibitory activity protein 3
UniProt Gene Name
MIA3
UniProt Synonym Gene Names
KIAA0268; TANGO; TANGO1
UniProt Entry Name
MIA3_HUMAN
Similar Products
Product Notes
The MIA3/TANGO mia3 (Catalog #AAA212980) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MIA3/TANGO Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MIA3/TANGO can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MIA3/TANGO mia3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIA3/TANGO, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.