WB (Western Blot)
(MIER1 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the MIER1 antibody detected the MIER1 protein (arrow).)
Rabbit MIER1 Polyclonal Antibody | anti-MIER1 antibody
MIER1 Antibody (Center)
Gene Names
MIER1; ER1; MI-ER1
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Flow Cytometry, Functional Assay, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Dot Blot, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MIER1, Antibody; MIER1 Antibody (Center); Mesoderm induction early response protein 1; Early response 1; Er1; Mi-er1; hMi-er1; MIER1; KIAA1610; anti-MIER1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MIER1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-242 amino acids from the Central region of human MIER1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
214-242
Sequence Length
81
Applicable Applications for anti-MIER1 antibody
FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), DB (Dot Blot), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
Human
Function
Transcriptional repressor regulating the expression of a number of genes including SP1 target genes. Probably functions through recruitment of HDAC1 a histone deacetylase involved in chromatin silencing.
Cellular location
[Isoform 1] Nucleus.[ Isoform3]: Nucleus.[Isoform 5]: Cytoplasm.
Tissue location
Ubiquitously expressed, but at very low levels. However, consistent level of expression are observed in heart, testis, thyroid, ovary and adrenal gland. Transcripts are up-regulated in breast carcinoma cell lines and tumor.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(MIER1 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the MIER1 antibody detected the MIER1 protein (arrow).)
WB (Western Blot)
(MIER1 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the MIER1 antibody detected the MIER1 protein (arrow).)
Related Product Information for anti-MIER1 antibody
Transcriptional repressor of a number of genes including Sp1 target genes.
References
McCarthy, P.L., et al. Br. J. Cancer 99(4):639-646(2008)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Nousiainen, M., et al. Proc. Natl. Acad. Sci. U.S.A. 103(14):5391-5396(2006)
Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57983
NCBI Official Full Name
mesoderm induction early response protein 1 isoform b
NCBI Official Synonym Full Names
mesoderm induction early response 1, transcriptional regulator
NCBI Official Symbol
MIER1
NCBI Official Synonym Symbols
ER1; MI-ER1
NCBI Protein Information
mesoderm induction early response protein 1
UniProt Protein Name
Mesoderm induction early response protein 1
UniProt Gene Name
MIER1
UniProt Synonym Gene Names
KIAA1610; Early response 1; Er1; Mi-er1; hMi-er1
UniProt Entry Name
MIER1_HUMAN
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Product Notes
The MIER1 mier1 (Catalog #AAA284792) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 214-242. The MIER1 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's MIER1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), DB (Dot Blot), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MIER1 mier1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIER1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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