IHC (Immunohiostchemistry)
(Anti-Human Milk Fat Globule 1 antibody, AAA45398, IHC(P)IHC(P): Human Mammary Cancer Tissue)
Rabbit anti-Human Milk Fat Globule 1 Polyclonal Antibody | anti-MFGE8 antibody
Anti-Human Milk Fat Globule 1 antibody
Gene Names
MFGE8; BA46; HMFG; MFGM; SED1; hP47; EDIL1; MFG-E8; SPAG10; OAcGD3S; HsT19888
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Milk Fat Globule 1, Antibody; Anti-Human Milk Fat Globule 1 antibody; Lactadherin; milk fat globule-EGF factor 8 protein; BA 46 antibody; BA46 antibody; Breast epithelial antigen BA46 antibody; Contains Medin antibody; EDIL 1 antibody; EDIL1 antibody; HMFG antibody; hP47 antibody; HsT19888 antibody; Lactadherin antibody; Lactadherin precursor antibody; Medin antibody; MFG E8 antibody; MFG-E8 antibody; MFGE 8 antibody; MFGE8 antibody; MFGE8 protein antibody; MFGM antibody; MFGM_HUMAN antibody; Milk fat globule EGF factor 8 antibody; Milk fat globule EGF factor 8 protein antibody; Milk fat globule-EGF factor 8 antibody; O acetyl disialoganglioside synthase antibody; OAcGD3S antibody; SED1 antibody; SPAG10 antibody; Sperm associated antigen 10 antibody; Sperm surface protein hP47 antibody; anti-MFGE8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
387
Applicable Applications for anti-MFGE8 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Human Milk Fat Globule 1 (165-180aa NGHEFDFIHDVNKKHK).
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Anti-Human Milk Fat Globule 1 antibody, AAA45398, IHC(P)IHC(P): Human Mammary Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti-Human Milk Fat Globule 1 antibody, AAA45398, IHC(P)IHC(P): Human Mammary Cancer Tissue)
WB (Western Blot)
(Anti-Human Milk Fat Globule 1 antibody, AAA45398, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant human MEGF8, 50.2KD(162aa tag+ S28-A317)Lane 1: Recombinant Human MEGF8 Protein 10ngLane 2: Recombinant Human MEGF8 Protein 5ngLane 3: Recombinant Human MEGF8 Protein 2.5ngLane 4: Recombinant Human MEGF8 Protein 1.25ng)
WB (Western Blot)
(Anti-Human Milk Fat Globule 1 antibody, AAA45398, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant human MEGF8, 50.2KD(162aa tag+ S28-A317)Lane 1: Recombinant Human MEGF8 Protein 10ngLane 2: Recombinant Human MEGF8 Protein 5ngLane 3: Recombinant Human MEGF8 Protein 2.5ngLane 4: Recombinant Human MEGF8 Protein 1.25ng)
Related Product Information for anti-MFGE8 antibody
Description: Rabbit IgG polyclonal antibody for Lactadherin(MFGE8) detection. Tested with WB, IHC-P in Human.
Background: MFGE8(Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al.(1997) mapped the MFGE8 gene to chromosome 15q25. Hanayama et al.(2002) found that MFGE8 is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al.(2007) showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon(PRKCE)-dependent mechanism.
Background: MFGE8(Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, Collins et al.(1997) mapped the MFGE8 gene to chromosome 15q25. Hanayama et al.(2002) found that MFGE8 is a factor that links apoptotic cells to phagocytes. MFGE8 specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. Bu et al.(2007) showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon(PRKCE)-dependent mechanism.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
37,523 Da
NCBI Official Full Name
Lactadherin
NCBI Official Synonym Full Names
milk fat globule-EGF factor 8 protein
NCBI Official Symbol
MFGE8
NCBI Official Synonym Symbols
BA46; HMFG; MFGM; SED1; hP47; EDIL1; MFG-E8; SPAG10; OAcGD3S; HsT19888
NCBI Protein Information
lactadherin; medin; sperm surface protein hP47; sperm associated antigen 10; breast epithelial antigen BA46; O-acetyl disialoganglioside synthase
UniProt Protein Name
Lactadherin
UniProt Gene Name
MFGE8
UniProt Synonym Gene Names
MFG-E8
UniProt Entry Name
MFGM_HUMAN
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Product Notes
The MFGE8 mfge8 (Catalog #AAA45398) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human Milk Fat Globule 1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Milk Fat Globule 1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MFGE8 mfge8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Milk Fat Globule 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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