WB (Western Blot)
(Anti-NR3C2 antibody, AAA45013, Western blottingLane 1: 293T Cell LysateLane 2: SMMC Cell LysateLane 3: SW620 Cell LysateLane 4: HELA Cell Lysate)
Rabbit Mineralocorticoid Receptor Polyclonal Antibody | anti-NR3C2 antibody
Anti-Mineralocorticoid Receptor antibody
Gene Names
NR3C2; MR; MCR; MLR; NR3C2VIT
Reactivity
Reacts with: HumanPredict to work with: Mouse, Rat
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
Mineralocorticoid Receptor, Antibody; Anti-Mineralocorticoid Receptor antibody; Mineralocorticoid receptor(MR); nuclear receptor subfamily 3, group C, member 2; Aldosterone receptor antibody; MCR antibody; MCR_HUMAN antibody; MGC133092 antibody; Mineralocorticoid receptor antibody; MLR antibody; MR antibody; NR3 C2 antibody; NR3C2 antibody; NR3C2 protein antibody; Nuclear receptor subfamily 3 group C member 2 antibody; anti-NR3C2 antibody
Host
Rabbit
Reactivity
Reacts with: Human
Predict to work with: Mouse, Rat
Predict to work with: Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
984
Applicable Applications for anti-NR3C2 antibody
WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Mineralocorticoid Receptor (966-984aa DQLPKVESGNAKPLYFHRK), identical to the related mouse and rat sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-NR3C2 antibody, AAA45013, Western blottingLane 1: 293T Cell LysateLane 2: SMMC Cell LysateLane 3: SW620 Cell LysateLane 4: HELA Cell Lysate)
WB (Western Blot)
(Anti-NR3C2 antibody, AAA45013, Western blottingLane 1: 293T Cell LysateLane 2: SMMC Cell LysateLane 3: SW620 Cell LysateLane 4: HELA Cell Lysate)
Related Product Information for anti-NR3C2 antibody
Description: Rabbit IgG polyclonal antibody for Mineralocorticoid receptor(NR3C2) detection. Tested with WB in Human, Mouse, Rat.
Background: NR3C2(nuclear receptor subfamily 3, group C, member 2) also known as MR(mineralocorticoid receptor) is a protein that in humans is encoded by the NR3C2 gene that is located on chromosome 4q31.1-31.2. NR3C2 is a receptor with high affinity for mineralocorticoids. It belongs to the nuclear receptor family where the ligand diffuses into cells, interacts with the receptor and results in a signal transduction affecting specific gene expression in the nucleus. MR is expressed in many tissues, such as the kidney, colon, heart, central nervous system(hippocampus), brown adipose tissue and sweat glands. In epithelial tissues, its activation leads to the expression of proteins regulating ionic and water transports(mainly the epithelial sodium channel or ENaC, Na+/K+ pump, serum and glucocorticoid induced kinase or SGK1) resulting in the reabsoprtion of sodium, and as a consequence an increase in extracellular volume, increase in blood pressure, and an excretion of potassium to maintain a normal salt concentration in the body. The receptor is activated by mineralocorticoids such as aldosterone and deoxycorticosterone as well as glucocorticoids, like cortisol. In intact animals, the mineralocorticoid receptor is "protected" from glucocorticoids by co-localization of an enzyme, 11beta-hydroxysteroid dehydrogenase type 2(11beta-HSD2), that converts cortisol to inactive cortisone. It also responds to some progestins. Spironolactone and eplerenone are mineralocorticoid receptor antagonists. Activation of the mineralocorticoid receptor, upon the binding of its ligand aldosterone, results in its translocation to the cell nucleus, homodimerization and binding to hormone response elements present in the promoter of some genes. This results in the complex recruitment of the transcriptional machinery and the transcription into mRNA of the DNA sequence of the activated genes.
Background: NR3C2(nuclear receptor subfamily 3, group C, member 2) also known as MR(mineralocorticoid receptor) is a protein that in humans is encoded by the NR3C2 gene that is located on chromosome 4q31.1-31.2. NR3C2 is a receptor with high affinity for mineralocorticoids. It belongs to the nuclear receptor family where the ligand diffuses into cells, interacts with the receptor and results in a signal transduction affecting specific gene expression in the nucleus. MR is expressed in many tissues, such as the kidney, colon, heart, central nervous system(hippocampus), brown adipose tissue and sweat glands. In epithelial tissues, its activation leads to the expression of proteins regulating ionic and water transports(mainly the epithelial sodium channel or ENaC, Na+/K+ pump, serum and glucocorticoid induced kinase or SGK1) resulting in the reabsoprtion of sodium, and as a consequence an increase in extracellular volume, increase in blood pressure, and an excretion of potassium to maintain a normal salt concentration in the body. The receptor is activated by mineralocorticoids such as aldosterone and deoxycorticosterone as well as glucocorticoids, like cortisol. In intact animals, the mineralocorticoid receptor is "protected" from glucocorticoids by co-localization of an enzyme, 11beta-hydroxysteroid dehydrogenase type 2(11beta-HSD2), that converts cortisol to inactive cortisone. It also responds to some progestins. Spironolactone and eplerenone are mineralocorticoid receptor antagonists. Activation of the mineralocorticoid receptor, upon the binding of its ligand aldosterone, results in its translocation to the cell nucleus, homodimerization and binding to hormone response elements present in the promoter of some genes. This results in the complex recruitment of the transcriptional machinery and the transcription into mRNA of the DNA sequence of the activated genes.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
94,359 Da
NCBI Official Full Name
Mineralocorticoid receptor
NCBI Official Synonym Full Names
nuclear receptor subfamily 3, group C, member 2
NCBI Official Symbol
NR3C2
NCBI Official Synonym Symbols
MR; MCR; MLR; NR3C2VIT
NCBI Protein Information
mineralocorticoid receptor; aldosterone receptor; mineralocorticoid receptor 1; mineralocorticoid receptor delta
UniProt Protein Name
Mineralocorticoid receptor
UniProt Gene Name
NR3C2
UniProt Synonym Gene Names
MCR; MLR; MR
UniProt Entry Name
MCR_HUMAN
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Product Notes
The NR3C2 nr3c2 (Catalog #AAA45013) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mineralocorticoid Receptor antibody reacts with Reacts with: Human Predict to work with: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Mineralocorticoid Receptor can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the NR3C2 nr3c2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mineralocorticoid Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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