WB (Western Blot)
(Detection of human MKL1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MKL1 antibody AAA211801 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. MKL1 was also immunoprecipitated by rabbit anti-MKL1 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
Rabbit anti-Human MKL1 Polyclonal Antibody | anti-MKL1 antibody
Rabbit anti-MKL1 Antibody, Affinity Purified
Gene Names
MKL1; MAL; BSAC; MRTF-A
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MKL1, Antibody; Rabbit anti-MKL1 Antibody, Affinity Purified; megakaryoblastic leukemia 1 protein; MRTF-A; MKL1; MKL/myocardin-like protein 1; megakaryocytic acute leukemia protein; megakaryoblastic leukemia (translocation) 1; MAL; BSAC; basic, SAP and coiled-coil domain; MKL; myocardin related transcription factor A; anti-MKL1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-MKL1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 700 and 750
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human MKL1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MKL1 antibody AAA211801 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. MKL1 was also immunoprecipitated by rabbit anti-MKL1 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human MKL1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MKL1 antibody AAA211801 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. MKL1 was also immunoprecipitated by rabbit anti-MKL1 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human MKL1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MKL1 (Cat. No. AAA211801 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human MKL1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MKL1 (Cat. No. AAA211801 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-MKL1 antibody
MKL1 (megakaryoblastic leukemia 1 protein) was identified as part of a t(1;22) translocation with RBM15 associated with acute megakaryoblastic leukemia. It is a transcriptional coactivator of serum response factor (SRF) that promotes muscle differentiation and also plays a role in promoting maturation of megakaryocytes.
Product Categories/Family for anti-MKL1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
98,919 Da
NCBI Official Full Name
MKL/myocardin-like protein 1
NCBI Official Synonym Full Names
megakaryoblastic leukemia (translocation) 1
NCBI Official Symbol
MKL1
NCBI Official Synonym Symbols
MAL; BSAC; MRTF-A
NCBI Protein Information
MKL/myocardin-like protein 1; basic, SAP and coiled-coil domain; megakaryoblastic leukemia 1 protein; megakaryocytic acute leukemia protein; myocardin-related transcription factor A; RNA-binding motif protein 15/megakaryoblastic leukemia-1 fusion protein
UniProt Protein Name
MKL/myocardin-like protein 1
UniProt Gene Name
MKL1
UniProt Synonym Gene Names
KIAA1438; MAL; MRTF-A
UniProt Entry Name
MKL1_HUMAN
Similar Products
Product Notes
The MKL1 mkl1 (Catalog #AAA211801) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MKL1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MKL1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MKL1 mkl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MKL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.