WB (Western Blot)
(MLL/HRX antibody (pAb) tested by Western blot. HeLa whole cell extract extract (20 ug) probed with MLL/HRX antibody (pAb) at 2 ug/ml.)
Rabbit anti-Human, Mouse MLL/HRX Polyclonal Antibody | anti-MLL/HRX antibody
MLL/HRX antibody (pAb)
Gene Names
KMT2A; HRX; MLL; MLL1; TRX1; ALL-1; CXXC7; HTRX1; MLL1A; WDSTS; MLL-AF9; MLL/GAS7; TET1-MLL
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein A Chromatography
Synonyms
MLL/HRX, Antibody; MLL/HRX antibody (pAb); KMT2A; MLL1; TRX1; ALL-1; CXXC7; HTRX1; MLL1A; WDSTS; MLL/GAS7; TET1-MLL; anti-MLL/HRX antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-MLL/HRX antibody
WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation)
Immunogen
This MLL/HRX antibody was raised against a recombinant protein corresponding to amino acids 2829-2883 of human MLL.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
WB (Western Blot)
(MLL/HRX antibody (pAb) tested by Western blot. HeLa whole cell extract extract (20 ug) probed with MLL/HRX antibody (pAb) at 2 ug/ml.)
WB (Western Blot)
(MLL/HRX antibody (pAb) tested by Western blot. HeLa whole cell extract extract (20 ug) probed with MLL/HRX antibody (pAb) at 2 ug/ml.)
IF (Immunofluorescence)
(MLL / HRX antibody (pAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with MLL / HRX antibody at a 0.5 ug/ml dilution.)
IF (Immunofluorescence)
(MLL / HRX antibody (pAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with MLL / HRX antibody at a 0.5 ug/ml dilution.)
ChIP (Chromatin Immunoprecipitation)
(MLL / HRX antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from HeLa cells and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify MLL / HRX binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
ChIP (Chromatin Immunoprecipitation)
(MLL / HRX antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from HeLa cells and 4 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 20 million sequence tags were mapped to identify MLL / HRX binding sites. The image shows binding across a region of chromosome 12. You can view the complete data set in the UCSC Genome Browser, starting at this specific location, here.)
Related Product Information for anti-MLL/HRX antibody
Short Description: Our MLL/HRX antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western blot; it has been shown to react with Human and Mouse samples.
Background: MLL (myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila)), also known as Histone-lysine N-methyltransferase HRX, is a histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1 /MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation. Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9'. Required for transcriptional activation of HOXA9. Promotes PPP1R15A-induced apoptosis.
Background: MLL (myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila)), also known as Histone-lysine N-methyltransferase HRX, is a histone methyltransferase that plays an essential role in early development and hematopoiesis. Catalytic subunit of the MLL1 /MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). In the MLL1/MLL complex, it specifically mediates H3K4me, a specific tag for epigenetic transcriptional activation. Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity. Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9'. Required for transcriptional activation of HOXA9. Promotes PPP1R15A-induced apoptosis.
Product Categories/Family for anti-MLL/HRX antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
432,052 Da
NCBI Official Full Name
histone-lysine N-methyltransferase 2A isoform 2
NCBI Official Synonym Full Names
lysine methyltransferase 2A
NCBI Official Symbol
KMT2A
NCBI Official Synonym Symbols
HRX; MLL; MLL1; TRX1; ALL-1; CXXC7; HTRX1; MLL1A; WDSTS; MLL-AF9; MLL/GAS7; TET1-MLL
NCBI Protein Information
histone-lysine N-methyltransferase 2A
UniProt Protein Name
Histone-lysine N-methyltransferase 2A
UniProt Gene Name
KMT2A
UniProt Synonym Gene Names
ALL1; CXXC7; HRX; HTRX; MLL; MLL1; TRX1; Lysine N-methyltransferase 2A; p320; p180
UniProt Entry Name
KMT2A_HUMAN
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Product Notes
The MLL/HRX kmt2a (Catalog #AAA60005) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MLL/HRX antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MLL/HRX can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), ChIP (Chromatin immunoprecipitation), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the MLL/HRX kmt2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLL/HRX, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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