WB (Western Blot)
(Detection of human MLLT1 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLLT1 antibody (AAA211824 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human MLLT1 Polyclonal Antibody | anti-MLLT1 antibody
Rabbit anti-MLLT1 Antibody, Affinity Purified
Gene Names
MLLT1; ENL; LTG19; YEATS1
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MLLT1, Antibody; Rabbit anti-MLLT1 Antibody, Affinity Purified; myeloid/lymphoid or mixed-lineage leukemia; translocated to, 1; YEATS1; protein ENL; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); MLL/ENL fusion protein; LTG19; ENL/MLL fusion; ENL; CTC-503J8.6; MLLT1/MLL fusion; YEATS domain-containing protein 1; Protein ENL; MLLT1, super elongation complex subunit; anti-MLLT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-MLLT1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 325 and 375
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human MLLT1 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLLT1 antibody (AAA211824 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human MLLT1 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLLT1 antibody (AAA211824 lot 2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human MLLT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MLLT1 antibody (AAA211824 lot 2) used for IP at 6 ug per reaction. MLLT1 was also immunoprecipitated by a previous lot of this antibody (AAA211824 lot 1) and a second antibody against a different epitope of MLLT1 . For blotting immunoprecipitated MLLT1, AAA211824 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human MLLT1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MLLT1 antibody (AAA211824 lot 2) used for IP at 6 ug per reaction. MLLT1 was also immunoprecipitated by a previous lot of this antibody (AAA211824 lot 1) and a second antibody against a different epitope of MLLT1 . For blotting immunoprecipitated MLLT1, AAA211824 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human MLLT1 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-MLLT1 (Cat. No. AAA211824 Lot2) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human MLLT1 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-MLLT1 (Cat. No. AAA211824 Lot2) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-MLLT1 antibody
MLLT1, also known as ENL (eleven nineteen leukemia protein), was originally identified as a gene involved in a recurrent translocation (11;19) with the mixed-lineage leukemia (MLL) gene. The MLL-ENL fusion protein has been found to associate with SWI/SNF complexes and promote transcription from the HoxA7 promoter. Additionally, from studies to identify proteins that associate with MLLT1, MLLT1 is suggested to play a role in histone modification and transcriptional elongation.
Product Categories/Family for anti-MLLT1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,056 Da
NCBI Official Full Name
protein ENL
NCBI Official Synonym Full Names
myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1
NCBI Official Symbol
MLLT1
NCBI Official Synonym Symbols
ENL; LTG19; YEATS1
NCBI Protein Information
protein ENL; CTC-503J8.6; ENL/MLL fusion; MLLT1/MLL fusion; YEATS domain-containing protein 1; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocated to, 1
UniProt Protein Name
Protein ENL
UniProt Gene Name
MLLT1
UniProt Synonym Gene Names
ENL; LTG19; YEATS1
UniProt Entry Name
ENL_HUMAN
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Product Notes
The MLLT1 mllt1 (Catalog #AAA211824) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MLLT1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MLLT1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MLLT1 mllt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLLT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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