WB (Western Blot)
(Western blot analysis of anti-MLLT1 Pab in A257 cell line lysate (35ug/lane). MLLT1(arrow) was detected using the purified Pab.)
Rabbit anti-Human MLLT1 Polyclonal Antibody | anti-MLLT1 antibody
MLLT1 Antibody (C-term)
Gene Names
MLLT1; ENL; LTG19; YEATS1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MLLT1, Antibody; MLLT1 Antibody (C-term); Protein ENL; YEATS domain-containing protein 1; MLLT1; ENL; LTG19; YEATS1; anti-MLLT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MLLT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 523-552 amino acids from the C-terminal region of human MLLT1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
523-552
Sequence Length
559
Applicable Applications for anti-MLLT1 antibody
ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of anti-MLLT1 Pab in A257 cell line lysate (35ug/lane). MLLT1(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-MLLT1 Pab in A257 cell line lysate (35ug/lane). MLLT1(arrow) was detected using the purified Pab.)
WB (Western Blot)
(All lanes : Anti-MLLT1 Antibody (D538) at 1:2000 dilutionLane 1: Raji whole cell lysatesLane 2: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-MLLT1 Antibody (D538) at 1:2000 dilutionLane 1: Raji whole cell lysatesLane 2: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-MLLT1 Antibody (D538)at 1:2000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-MLLT1 Antibody (D538)at 1:2000 dilution + Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 62 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-MLLT1 antibody
Chromosome band 11q23 is the site of translocations in myeloid and lymphoid acute leukemias, pediatric leukemias, and treatment-induced secondary acute myelogenous leukemia. The translocation breakpoints cluster in a restricted region of the HRX gene resulting in chimeric genes that encode an N-terminal portion of Hrx fused to various partner proteins. Myeloid/lymphoid or mixed-lineage leukemia translocated to 1 (MLLT1) is a nuclear protein with transcriptional transactivation properties that is fused to Hrx in t(11;19) leukemias. The minimal MLLT1 sequence required for transcription activation was narrowed to the C-terminal 90 amino acids.
References
Nie, Z., et al., Mol. Cell. Biol. 23(8):2942-2952 (2003).
Lavau, C., et al., Proc. Natl. Acad. Sci. U.S.A. 97(20):10984-10989 (2000).
Thirman, M.J., et al., Proc. Natl. Acad. Sci. U.S.A. 91(25):12110-12114 (1994).
Rubnitz, J.E., et al., Blood 84(6):1747-1752 (1994).
Yamamoto, K., et al., Oncogene 8(10):2617-2625 (1993).
Lavau, C., et al., Proc. Natl. Acad. Sci. U.S.A. 97(20):10984-10989 (2000).
Thirman, M.J., et al., Proc. Natl. Acad. Sci. U.S.A. 91(25):12110-12114 (1994).
Rubnitz, J.E., et al., Blood 84(6):1747-1752 (1994).
Yamamoto, K., et al., Oncogene 8(10):2617-2625 (1993).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62056
NCBI Official Full Name
protein ENL
NCBI Official Synonym Full Names
myeloid/lymphoid or mixed-lineage leukemia; translocated to, 1
NCBI Official Symbol
MLLT1
NCBI Official Synonym Symbols
ENL; LTG19; YEATS1
NCBI Protein Information
protein ENL
UniProt Protein Name
Protein ENL
UniProt Gene Name
MLLT1
UniProt Synonym Gene Names
ENL; LTG19; YEATS1
UniProt Entry Name
ENL_HUMAN
Similar Products
Product Notes
The MLLT1 mllt1 (Catalog #AAA284562) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 523-552. The MLLT1 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MLLT1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MLLT1 mllt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLLT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.