IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with MLLT10 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit anti-Human MLLT10 (AF10) Polyclonal Antibody | anti-MLLT10 antibody
MLLT10 (AF10) Antibody (C-term)
Gene Names
MLLT10; AF10
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MLLT10 (AF10), Antibody; MLLT10 (AF10) Antibody (C-term); Protein AF-10; ALL1-fused gene from chromosome 10 protein; MLLT10; AF10; anti-MLLT10 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MLLT10 (AF10) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 998-1027 amino acids from the C-terminal region of human MLLT10 (AF10).
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
998-1027
Sequence Length
179
Applicable Applications for anti-MLLT10 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with MLLT10 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with MLLT10 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of anti-MLLT10 Pab in Jurkat cell line lysate. MLLT10(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-MLLT10 Pab in Jurkat cell line lysate. MLLT10(arrow) was detected using the purified Pab.)
Related Product Information for anti-MLLT10 antibody
Translocations affecting chromosome 11q23 involve many partner chromosome regions and occur in various leukemias. The 11q23 gene involved in the translocations is MLL. MLLT10 is the partner gene to MLL1 involved in t(10;11)(p12;q23) translocations. In an analysis of two leukemia patients, the in t(10;11)(p12;q23) translocation fuses MLL1, a SET domain containg histone methyltransferase, to the MLLT10 gene. The MLLT10 gene encodes a predicted 1,027-amino acid protein containing an N-terminal zinc finger and a C-terminal leucine zipper domain. The MLLT10 gene is one of the few MLL partner genes to be independently rearranged with a third gene in leukemia, the CALM gene in the t(10;11)(p12;q14) translocation. Chimeric fusion proteins MLL/AF10 and CALM/AF10 consistently retain the leucine zipper motif of MLLT10. The leucine zipper interacts with GAS41, a protein previously identified as the product of an amplified gene in a glioblastoma. GAS41 interacts with integrase interactor-1 (INI1), a component of the SWI/SNF complex, which acts to remodel chromatin and to modulate transcription. Retention of the leucine zipper in the MLL and CALM fusions suggested that a key feature of these chimeric proteins may be their ability to interfere in normal gene regulation through interaction with the adenosine triphosphate-dependent chromatin remodeling complexes.
References
Perrin, L., et al., Mol. Cell. Biol. 23(1):119-130 (2003).
Nakamura, T., et al., Mol. Cell 10(5):1119-1128 (2002).
Roll, P., et al., Cancer Genet. Cytogenet. 135(2):187-191 (2002).
Chaplin, T., et al., Blood 85(6):1435-1441 (1995).
Nakamura, T., et al., Mol. Cell 10(5):1119-1128 (2002).
Roll, P., et al., Cancer Genet. Cytogenet. 135(2):187-191 (2002).
Chaplin, T., et al., Blood 85(6):1435-1441 (1995).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
113320
NCBI Official Full Name
protein AF-10 isoform c
NCBI Official Synonym Full Names
myeloid/lymphoid or mixed-lineage leukemia; translocated to, 10
NCBI Official Symbol
MLLT10
NCBI Official Synonym Symbols
AF10
NCBI Protein Information
protein AF-10
UniProt Protein Name
Protein AF-10
UniProt Gene Name
MLLT10
UniProt Synonym Gene Names
AF10
UniProt Entry Name
AF10_HUMAN
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Product Notes
The MLLT10 mllt10 (Catalog #AAA290236) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 998-1027. The MLLT10 (AF10) Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MLLT10 (AF10) can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MLLT10 mllt10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLLT10 (AF10), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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