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WB (Western Blot)
(MMP14 Antibody (R160) (Cat.# AAA284118) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MMP14 antibody detected the MMP14 protein (arrow).)
Rabbit MMP14 Polyclonal Antibody | anti-MMP14 antibody
MMP14 Antibody (N-term)
Gene Names
MMP14; MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
Reactivity
Human, MousePredicted: Bovine, Pig, Rat
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MMP14, Antibody; MMP14 Antibody (N-term); Matrix metalloproteinase-14; MMP-14; MMP-X1; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; MT1-MMP; MT1MMP; MMP14; anti-MMP14 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted: Bovine, Pig, Rat
Predicted: Bovine, Pig, Rat
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MMP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-174 amino acids from the N-terminal region of human MMP14.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBSwith 0.09% (W/V) sodium azide. This antibodyis purified through a protein A column,followed by peptide affinity purification.
Sequence Positions
145-174
Sequence Length
582
Applicable Applications for anti-MMP14 antibody
ELISA, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry)
Antigen Source
HUMAN
Function
Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues in association with pro- MMP2.
Cellular Location
Membrane; Single-pass type I membrane protein. Melanosome. Cytoplasm Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm
Tissue Location
Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(MMP14 Antibody (R160) (Cat.# AAA284118) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MMP14 antibody detected the MMP14 protein (arrow).)
WB (Western Blot)
(MMP14 Antibody (R160) (Cat.# AAA284118) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the MMP14 antibody detected the MMP14 protein (arrow).)
WB (Western Blot)
(All lanes : Anti-MMP14 Antibody (R160) at 1:2000 dilution:Lane 1: A2058 whole cell lysateLane 2: HT-1080 whole cell lysateLane 3: mouse lung lysateLane 4: MCF-7 whole cell lysateLane 5: NCI-H292 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 66 kDaBlocking/Dilution buffer: 5% NFDM/TBST)
WB (Western Blot)
(All lanes : Anti-MMP14 Antibody (R160) at 1:2000 dilution:Lane 1: A2058 whole cell lysateLane 2: HT-1080 whole cell lysateLane 3: mouse lung lysateLane 4: MCF-7 whole cell lysateLane 5: NCI-H292 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 66 kDaBlocking/Dilution buffer: 5% NFDM/TBST)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells using MMP14 Antibody (N-term) (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells using MMP14 Antibody (N-term) (bottom histogram) compared to a negative control (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemistry)
(AAA284118 staining MMP14 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
IHC (Immunohistochemistry)
(AAA284118 staining MMP14 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Related Product Information for anti-MMP14 antibody
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP14 seems to specifically activate progelatinase A, and may thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. Expression is significant in stromal cells of colon, breast, and head and neck.
Product Categories/Family for anti-MMP14 antibody
References
Will, H., et al., Eur. J. Biochem. 231(3):602-608 (1995).
Takino, T., et al., Gene 155(2):293-298 (1995).
Okada, A., et al., Proc. Natl. Acad. Sci. U.S.A. 92(7):2730-2734 (1995).
Sato, H., et al., Nature 370(6484):61-65 (1994).
Takino, T., et al., Gene 155(2):293-298 (1995).
Okada, A., et al., Proc. Natl. Acad. Sci. U.S.A. 92(7):2730-2734 (1995).
Sato, H., et al., Nature 370(6484):61-65 (1994).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
matrix metalloproteinase-14 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 14 (membrane-inserted)
NCBI Official Symbol
MMP14
NCBI Official Synonym Symbols
MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
NCBI Protein Information
matrix metalloproteinase-14
UniProt Protein Name
Matrix metalloproteinase-14
UniProt Gene Name
MMP14
UniProt Synonym Gene Names
MMP-14; MT-MMP 1; MTMMP1; MT1-MMP; MT1MMP
UniProt Entry Name
MMP14_HUMAN
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Product Notes
The MMP14 mmp14 (Catalog #AAA284118) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 145-174. The MMP14 Antibody (N-term) reacts with Human, Mouse Predicted: Bovine, Pig, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MMP14 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the MMP14 mmp14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMP14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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