Rabbit MMP9 / Gelatinase B Polyclonal Antibody | anti-MMP9 antibody

Rabbit Polyclonal to Human MMP9 / Gelatinase B

Cat. Num. AAA50685

• Gene Names: MMP9; GELB; CLG4B; MMP-9; MANDP2
• Reactivity: Human, Bovine; Predicted: Monkey, Dog, Hamster, Rabbit
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: MMP9 / Gelatinase B, Antibody; Rabbit Polyclonal to Human MMP9 / Gelatinase B; Anti-MMP9 / Gelatinase B Antibody (Internal) IHC-plus; MMP9; 92kd gelatinase; 92 kd type iv collagenase; 92 kDa gelatinase; 92 kDa type IV collagenase; Matrix metalloproteinase-9; MMP-9; Type V collagenase; CLG4B; Gelatinase B; GELB; Macrophage gelatinase; MANDP2; Human MMP9; anti-MMP9 antibody

• Host: Rabbit
• Reactivity: Human, Bovine; Predicted: Monkey, Dog, Hamster, Rabbit
• Clonality: Polyclonal
• Specificity: Human MMP9. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 0.4 mg/ml (varies by lot)
• Sequence Length: 707

• Applicable Applications for anti-MMP9 antibody: IHC (Immunohistochemistry)
• Target Species: Human
• Immunogen Description: Synthetic 12 amino acid peptide from internal region of human MMP9. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Bovine (100%); Monkey, Dog, Hamster, Rabbit (92%); Mouse, Panda, Horse, Pig, Chicken (83%).
• Immunogen Type: Synthetic peptide
• Immunogen: MMP9 / Gelatinase B antibody was raised against synthetic 12 amino acid peptide from internal region of human MMP9. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Bovine (100%); Monkey, Dog, Hamster, Rabbit (92%); Mouse, Panda, Horse, Pig, Chicken (83%).
• Epitope: Internal
• Immunogen Species: MMP9 / Gelatinase B antibody was raised against Human
• Antigen Type: Synthetic peptide
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store undiluted at -20 degree C or below for to 1 year. Can be stored undiluted at 4 degree C for up to 1 month, Avoid freeze/thaw cycles

IHC (Immunohistochemistry)

(Anti-MMP9 antibody IHC of human skin, granulation tissue. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-MMP9 antibody

Matrix metalloproteinases (MMPs) are neutral proteinases that are involved in the breakdown and remodeling of the extracellular matrix (ECM) under a variety of physiological and pathological conditions. MMP9 is a Metallopeptidase M10A type protease and is particularly important in the pathogenesis of inflammatory, infectious, and neoplastic diseases in many organs.

Product Categories/Family for anti-MMP9 antibody

Family: Protease; Subfamily: Metallopeptidase M10A

References

Altered Microenvironment Promotes Progression of Preinvasive Breast Cancer: Myoepithelial Expression of ?v?6 Integrin in DCIS Identifies High-risk Patients and Predicts Recurrence. Michael D. Allen, Gareth J. Thomas, Sarah Clark, Marwa M. Dawoud, Sabarinath Vallath, Sarah J. Payne, Jennifer J. Gomm, Sally A. Dreger, Sarah Dickinson, Dylan R. Edwards, Caroline J. Pennington, Ivana Sestak, Jack Cuzick, John F. Marshall, Ian R. Hart and J. Louise Jones (2014)

NCBI and Uniprot Product Information

• NCBI GI #: 74272287
• NCBI GeneID: 4318
• NCBI Accession #: NP_004985.2
• NCBI GenBank Nucleotide #: NM_004994.2
• UniProt Accession #: P14780; Q3LR70; Q8N725; Q9H4Z1; Q9UCJ9; Q9UCL1; Q9UDK2; B2R7V9
• NCBI Official Full Name: matrix metalloproteinase-9 preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• NCBI Official Symbol: MMP9
• NCBI Official Synonym Symbols: GELB; CLG4B; MMP-9; MANDP2
• NCBI Protein Information: matrix metalloproteinase-9; type V collagenase; macrophage gelatinase; matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
• UniProt Protein Name: Matrix metalloproteinase-9
• UniProt Gene Name: MMP9
• UniProt Synonym Gene Names: CLG4B; MMP-9; GELB
• UniProt Entry Name: MMP9_HUMAN