WB (Western Blot)
(Detection of human MMS21 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MMS21 antibody AAA212509 (lot AAA212509-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human MMS21 Polyclonal Antibody | anti-MMS21 antibody
Rabbit anti-MMS21 Antibody, Affinity Purified
Gene Names
NSMCE2; NSE2; MMS21; ZMIZ7; C8orf36
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MMS21, Antibody; Rabbit anti-MMS21 Antibody, Affinity Purified; non-SMC element 2, MMS21 homolog; ZMIZ7; zinc finger, MIZ-type containing 7; PVT1/NSMCE2 fusion; NSMCE2/PVT1 fusion; NSE2; non-structural maintenance of chromosomes element 2 homolog; MMS21; MMS21 homolog; C8orf36; methyl methanesulfonate sensitivity gene 21; hMMS21; E3 SUMO-protein transferase NSE2; E3 SUMO-protein ligase NSE2; Non-SMC element 2 homolog; NSE2 (MMS21) homolog, SMC5-SMC6 complex SUMO ligase; anti-MMS21 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MMS21 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human MMS21 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MMS21 antibody AAA212509 (lot AAA212509-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human MMS21 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MMS21 antibody AAA212509 (lot AAA212509-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human MMS21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MMS21 antibody AAA212509 (lot AAA212509-2) used for IP at 6 ug per reaction. MMS21 was also immunoprecipitated by a previous lot of this antibody (lot AAA212509-1) and rabbit anti-MMS21 antibody For blotting immunoprecipitated MMS21, AAA212509 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human MMS21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MMS21 antibody AAA212509 (lot AAA212509-2) used for IP at 6 ug per reaction. MMS21 was also immunoprecipitated by a previous lot of this antibody (lot AAA212509-1) and rabbit anti-MMS21 antibody For blotting immunoprecipitated MMS21, AAA212509 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Related Product Information for anti-MMS21 antibody
The human MMS21 (Methyl Methanesulfonate-Sensitivity protein 21) homolog is also known as E3 SUMO-protein ligase NSE2. MMS21 is an E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96MF7].
Product Categories/Family for anti-MMS21 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,932 Da
NCBI Official Full Name
E3 SUMO-protein ligase NSE2 isoform 1
NCBI Official Synonym Full Names
NSE2 (MMS21) homolog, SMC5-SMC6 complex SUMO ligase
NCBI Official Symbol
NSMCE2
NCBI Official Synonym Symbols
NSE2; MMS21; ZMIZ7; C8orf36
NCBI Protein Information
E3 SUMO-protein ligase NSE2
UniProt Protein Name
E3 SUMO-protein ligase NSE2
UniProt Gene Name
NSMCE2
UniProt Synonym Gene Names
C8orf36; MMS21; hMMS21; Non-SMC element 2 homolog
UniProt Entry Name
NSE2_HUMAN
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Product Notes
The MMS21 nsmce2 (Catalog #AAA212509) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MMS21 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MMS21 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MMS21 nsmce2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MMS21, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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