Rabbit anti-Human MOB kinase activator 1A Polyclonal Antibody | anti-MOB1A antibody

Rabbit anti-human MOB kinase activator 1A polyclonal Antibody

Cat. Num. AAA230011

• Gene Names: MOB1A; MOB1; MATS1; Mob4B; C2orf6; MOBK1B; MOBKL1B
• Reactivity: Human
• Applications: Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: >95%, Protein G purified
• Synonyms: MOB kinase activator 1A, Antibody; Rabbit anti-human MOB kinase activator 1A polyclonal Antibody; Mob1 alpha; Mob1 homolog 1B; Mps one binder kinase activator-like 1B; C2orf6; MOB4B; MOBK1B; MOBKL1B; MOB1A; anti-MOB1A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 216

• Applicable Applications for anti-MOB1A antibody: IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: Recombinant human MOB kinase activator 1A protein (2-216AA).
• Species: Homo sapiens (Human)
• Research Area: Signal Transduction
• Target Names: MOB1A
• Conjugation: Non-conjugated
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-161867
• Preparation and Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

IP (Immunoprecipitation)

(Immunoprecipitating MOB1A in Jurkat whole cell lysateLane 1: Rabbit control IgG instead of AAA230011 in Jurkat whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA230011 (5ug) + Jurkat whole cell lysate (500ug)Lane 3: Jurkat whole cell lysate (20ug))

IF (Immunofluorescence)

(Immunofluorescence staining of HepG2 cells with AAA230011 at 1:333, counter- stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

IHC (Immunohistochemisry)

(IHC image of AAA230011 diluted at 1:1000 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

WB (Western Blot)

(Western BlotPositive WB detected in: K562 whole cell lysate, Jurkat whole cell lysateAll lanes: MOB1A antibody at 3.3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 26, 17 kDaObserved band size: 26 kDa)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human small intestine tissue using AAA230011 at dilution of 1:100)

Related Product Information for anti-MOB1A antibody

Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L. Acts cooperatively with STK3/MST2 to activate STK38.

NCBI and Uniprot Product Information

• NCBI GI #: 148747437
• NCBI GeneID: 55233
• NCBI Accession #: NP_060691.2
• NCBI GenBank Nucleotide #: NM_018221.4
• UniProt Accession #: Q9H8S9; Q53S34; Q9H3T5; Q9HAI0; Q9NVE2
• NCBI Official Full Name: MOB kinase activator 1A isoform 1
• NCBI Official Synonym Full Names: MOB kinase activator 1A
• NCBI Official Symbol: MOB1A
• NCBI Official Synonym Symbols: MOB1; MATS1; Mob4B; C2orf6; MOBK1B; MOBKL1B
• NCBI Protein Information: MOB kinase activator 1A
• UniProt Protein Name: MOB kinase activator 1A
• UniProt Gene Name: MOB1A
• UniProt Synonym Gene Names: C2orf6; MOB4B; MOBK1B; MOBKL1B; Mob1A
• UniProt Entry Name: MOB1A_HUMAN