IHC (Immunohistochemistry)
(Rat Skeletal Muscle- MOTS-c Primary Antibody: AAA14413; 1:50 dilution in IHC Blocking Buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification on Leica DM4000. FFPE section.)
Rabbit MOTS-c Polyclonal Antibody | anti-MOTS-c antibody
Rabbit Polyclonal Anti-MOTS-c antibody
Applications
ELISA, Immunoprecipitation, Western Blot, Dot Blot
Synonyms
MOTS-c, Antibody; Rabbit Polyclonal Anti-MOTS-c antibody; Mitochondrial Derived Peptide MOTS-c; MOTS; MOTS c; anti-MOTS-c antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Form/Format
Affinity Purified Immunoglobulins
Concentration
0.75 mg/ml IgG in antibody stabilization buffer (varies by lot)
Sequence Length
16
Applicable Applications for anti-MOTS-c antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB), Dot Blot (DB)
Application Notes
Dot Blot: 1:10,000
ELISA: 1:10,000
Immunoprecipitation: 1:200
Western Blot: 1:500
Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigations intending to use an application that has not been verified can request a complimentary sample.
ELISA: 1:10,000
Immunoprecipitation: 1:200
Western Blot: 1:500
Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigations intending to use an application that has not been verified can request a complimentary sample.
Immunogen
Full-length peptide corresponding to amino acids 1-16 on MOTS-c.
Species Cross Reactivity
Human
Preparation and Storage
Store at -20°C for long term storage.
IHC (Immunohistochemistry)
(Rat Skeletal Muscle- MOTS-c Primary Antibody: AAA14413; 1:50 dilution in IHC Blocking Buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification on Leica DM4000. FFPE section.)
IHC (Immunohistochemistry)
(Rat Skeletal Muscle- MOTS-c Primary Antibody: AAA14413; 1:50 dilution in IHC Blocking Buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification on Leica DM4000. FFPE section.)
WB (Western Blot)
(Western Blot of AAA14413 with HepG2 cells.)
WB (Western Blot)
(Western Blot of AAA14413 with HepG2 cells.)
Related Product Information for anti-MOTS-c antibody
Description: Affinity Purified Mitochondrial-Derived Peptide MOTS-c Antibody
Overview: Mitochondria are functioning organelles that play a secondary role in signaling within the cell. While the details of mitochondrial signaling are not fully understood, the identification of an open reading frame in the mitochondrial DNA coding for humanin suggests that there are more signaling peptides coded by portions of the mtDNA. A short open reading frame within the 12S subunit of the mitochondrial ribosomal RNA codes for a 16 amino acid peptide called mitochondrial open reading frame of 12S rRNA-c, or MOTS-c. MOTS-c plays a role in insulin sensitivity and metabolic homeostasis (1). MOTS-c targets skeletal muscle tissue, and the mechanism of its action involves inhibition of the folate cycle. MOTS-c promotes the formation of an endogenous AMP analog called 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR). AICAR is an activator of AMPK, suggesting that MOTS-c could be used in an insulin-regulation capacity in order to treat type 2 diabetes (2).
MOTS-c selective antibodies were generated against a peptide taken from the human protein. The MOTS-c-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The MOTS-c-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The lab provides MOTS-c Western blot positive control samples in “ready-to-use” SDS-PAGE sample buffer. Antigenic blocking peptide for MOTS-c antibody is also available, please inquire before ordering. The lab can conjugate this antibody to fluorophores and other secondary enzymes as an additional service.
Overview: Mitochondria are functioning organelles that play a secondary role in signaling within the cell. While the details of mitochondrial signaling are not fully understood, the identification of an open reading frame in the mitochondrial DNA coding for humanin suggests that there are more signaling peptides coded by portions of the mtDNA. A short open reading frame within the 12S subunit of the mitochondrial ribosomal RNA codes for a 16 amino acid peptide called mitochondrial open reading frame of 12S rRNA-c, or MOTS-c. MOTS-c plays a role in insulin sensitivity and metabolic homeostasis (1). MOTS-c targets skeletal muscle tissue, and the mechanism of its action involves inhibition of the folate cycle. MOTS-c promotes the formation of an endogenous AMP analog called 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR). AICAR is an activator of AMPK, suggesting that MOTS-c could be used in an insulin-regulation capacity in order to treat type 2 diabetes (2).
MOTS-c selective antibodies were generated against a peptide taken from the human protein. The MOTS-c-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The MOTS-c-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The lab provides MOTS-c Western blot positive control samples in “ready-to-use” SDS-PAGE sample buffer. Antigenic blocking peptide for MOTS-c antibody is also available, please inquire before ordering. The lab can conjugate this antibody to fluorophores and other secondary enzymes as an additional service.
Product Categories/Family for anti-MOTS-c antibody
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
NCBI Official Full Name
MOTS-c
UniProt Protein Name
Mitochondrial-derived peptide MOTS-c
UniProt Gene Name
MT-RNR1
UniProt Entry Name
MOTSC_HUMAN
Similar Products
Product Notes
The MOTS-c mt-rnr1 (Catalog #AAA14413) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MOTS-c can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB), Dot Blot (DB). Dot Blot: 1:10,000 ELISA: 1:10,000 Immunoprecipitation: 1:200 Western Blot: 1:500 Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigations intending to use an application that has not been verified can request a complimentary sample. Researchers should empirically determine the suitability of the MOTS-c mt-rnr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MOTS-c, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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