Rabbit anti-Human MRPS10 Polyclonal Antibody | anti-MRPS10 antibody

Anti-MRPS10 Antibody, Rabbit Polyclonal

Cat. Num. AAA256099

• Reactivity: Human
• Applications: Immunoprecipitation, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: MRPS10, Antibody; Anti-MRPS10 Antibody, Rabbit Polyclonal; MRPS10 Antibody, Rabbit PAb, Antigen Affinity Purified; mitochondrial ribosomal protein S10; Anti-MRP-S10 Antibody; Anti-PNAS-122 Antibody; anti-MRPS10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MRPS10
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-MRPS10 antibody: IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: E Coli-derived Human MRPS10 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human MRPS10 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(MRPS10 was immunoprecipitated using:Lane A:0.5 mg U-251 MG MDA-MB453 Whole Cell Lysate4 uL anti-MRPS10 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MRPS10 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size :18 kDa)

WB (Western Blot)

(Anti-MRPS10 rabbit polyclonal antibody at 1:500 dilutionLane A: MDA-MB453 Whole Cell LysateLane B: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:19 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-MRPS10 antibody

MRPS10 (Mitochondrial Ribosomal Protein S10) is a Protein Coding gene. The MRPS10 gene, located on 6p21.1, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, and frog. MRPS10 belongs to the universal ribosomal protein uS10 family. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S10P family. Pseudogenes corresponding to this gene are found on chromosomes 1q, 3p, and 9p. MRPS10 is widely expressed in the esophagus, heart, and other tissues. Among its related pathways are Organelle biogenesis and maintenance and Viral mRNA Translation. 247 organisms have orthologs with the human gene MRPS10.

References

Yagi M, et al. (2012) P32/gc1qr is indispensable for fetal development and mitochondrial translation: Importance of its rna-binding ability. Nucleic Acids Res 40 (19): 9717-9737.Koc EC, et al. (2000) A proteomics approach to the identification of mammalian mitochondrial small subunit ribosomal proteins. J Biol Chem 275 (42): 32585-32591.Jia L, et al. (2002) Gene expression profile of human bone marrow stromal cells: High-throughput expressed sequence tag sequencing analysis. Genomics 79 (1): 7-17.