WB (Western Blot)
(Figure 2. Western blot analysis of MST3/STK24 using anti-MST3/STK24 antibody (AAA126352).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat stomach tissue lysates,Lane 2: rat testis tissue lysates,Lane 3: rat brain tissue lysates,Lane 4: mouse stomach tissue lysates,Lane 5: mouse small intestin tissue lysates,Lane 6: mouse testis tissue lysates,Lane 7: mouse brian tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST3/STK24 antigen affinity purified polyclonal antibody (#AAA126352) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MST3/STK24 at approximately 55 kDa. The expected band size for MST3/STK24 is at 49 kDa.)
Rabbit MST3/STK24 Polyclonal Antibody | anti-STK24 antibody
Anti-MST3/STK24 Antibody Picoband
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
WB (Western Blot)
(Figure 2. Western blot analysis of MST3/STK24 using anti-MST3/STK24 antibody (AAA126352).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat stomach tissue lysates,Lane 2: rat testis tissue lysates,Lane 3: rat brain tissue lysates,Lane 4: mouse stomach tissue lysates,Lane 5: mouse small intestin tissue lysates,Lane 6: mouse testis tissue lysates,Lane 7: mouse brian tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST3/STK24 antigen affinity purified polyclonal antibody (#AAA126352) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MST3/STK24 at approximately 55 kDa. The expected band size for MST3/STK24 is at 49 kDa.)
WB (Western Blot)
(Figure 2. Western blot analysis of MST3/STK24 using anti-MST3/STK24 antibody (AAA126352).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat stomach tissue lysates,Lane 2: rat testis tissue lysates,Lane 3: rat brain tissue lysates,Lane 4: mouse stomach tissue lysates,Lane 5: mouse small intestin tissue lysates,Lane 6: mouse testis tissue lysates,Lane 7: mouse brian tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST3/STK24 antigen affinity purified polyclonal antibody (#AAA126352) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MST3/STK24 at approximately 55 kDa. The expected band size for MST3/STK24 is at 49 kDa.)
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of U251 cells using anti-MST3/STK24 antibody (AAA126352).Overlay histogram showing U251 cells stained with AAA126352 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MST3/STK24 Antibody (AAA126352, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 5. Flow Cytometry analysis of U251 cells using anti-MST3/STK24 antibody (AAA126352).Overlay histogram showing U251 cells stained with AAA126352 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MST3/STK24 Antibody (AAA126352, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IHC (Immunohistochemisry)
(Figure 4. IHC analysis of MST3/STK24 using anti-MST3/STK24 antibody (AAA126352).MST3/STK24 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MST3/STK24 Antibody (AAA126352) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemisry)
(Figure 4. IHC analysis of MST3/STK24 using anti-MST3/STK24 antibody (AAA126352).MST3/STK24 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MST3/STK24 Antibody (AAA126352) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of MST3/STK24 using anti-MST3/STK24 antibody (AAA126352).MST3/STK24 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MST3/STK24 Antibody (AAA126352) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohiostchemistry)
(Figure 3. IHC analysis of MST3/STK24 using anti-MST3/STK24 antibody (AAA126352).MST3/STK24 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MST3/STK24 Antibody (AAA126352) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
WB (Western Blot)
(Figure 1. Western blot analysis of MST3/STK24 using anti-MST3/STK24 antibody (AAA126352).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A431 whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human Raji whole cell lysates,Lane 4: human U-87MG whole cell lysates,Lane 5: human 293T whole cell lysates,Lane 6: human placenta tissue lysates,Lane 7: human CACO-2 whole cell lysates,Lane 8: human RT4 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST3/STK24 antigen affinity purified polyclonal antibody (#AAA126352) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MST3/STK24 at approximately 55 kDa. The expected band size for MST3/STK24 is at 49 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of MST3/STK24 using anti-MST3/STK24 antibody (AAA126352).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A431 whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human Raji whole cell lysates,Lane 4: human U-87MG whole cell lysates,Lane 5: human 293T whole cell lysates,Lane 6: human placenta tissue lysates,Lane 7: human CACO-2 whole cell lysates,Lane 8: human RT4 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MST3/STK24 antigen affinity purified polyclonal antibody (#AAA126352) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MST3/STK24 at approximately 55 kDa. The expected band size for MST3/STK24 is at 49 kDa.)
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Product Notes
The STK24 stk24 (Catalog #AAA126352) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MST3/STK24 Antibody Picoband reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MST3/STK24 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the STK24 stk24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MST3/STK24, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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