Rabbit anti-Mouse, Rat MTCO1 Polyclonal Antibody | anti-MT-CO1 antibody

Anti-MTCO1 antibody

Cat. Num. AAA45740

• Reactivity: Mouse, Rat
• Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: MTCO1, Antibody; Anti-MTCO1 antibody; Cytochrome c oxidase subunit 1; mitochondrially encoded cytochrome c oxidase I; COI antibody; COX I antibody; COX1_HUMAN antibody; COXI antibody; Cytochrome c oxidase polypeptide I antibody; Cytochrome c oxidase subunit 1 antibody; Cytochrome C Oxidase subunit I antibody; Mitochondrially encoded cytochrome c oxidase I antibody; MT CO1 antibody; MT-CO1 antibody; MTCO 1 antibody; MTCO1 antibody; anti-MT-CO1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 514

• Applicable Applications for anti-MT-CO1 antibody: ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse MTCO1 (501-514aa PYHTFEEPTYVKVK), different from the related rat sequence by one amino acid.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

ICC (Immunocytochemistry)

(Anti-MTCO1 antibody, AAA45740, ICCICC: HEPA Cell)

IHC (Immunohistochemistry)

(Anti-MTCO1 antibody, AAA45740, IHC(P)IHC(P): Mouse Spleen Tissue)

IHC (Immunohistochemisry)

(Anti-MTCO1 antibody, AAA45740, IHC(P)IHC(P): Mouse Liver Tissue)

IHC (Immunohiostchemistry)

(Anti-MTCO1 antibody, AAA45740, IHC(P)IHC(P): Rat Brain Tissue)

WB (Western Blot)

(Anti-MTCO1 antibody, AAA45740, Western blottingLane 1: Rat Heart Tissue LysateLane 2: Mouse Heart Tissue Lysate)

Related Product Information for anti-MT-CO1 antibody

Background: Cytochrome c oxidase subunit I(CO1 or MTCO1) is 1 of 3 mitochondrial DNA(mtDNA) encoded subunits(MTCO1, MTCO2, MTCO3) of respiratory Complex IV. Complex IV is located within the mitochondrial inner membrane and is the third and final enzyme of the electron transport chain of mitochondrial oxidative phosphorylation. It is composed of 13 polypeptides. Subunits I, II, and III(MTCO1, MTCO2, MTCO3) are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc, and VIII are nuclear encoded. The cytochrome c oxidase family of enzymes have 4 redox centers, 2 hemes and 2 copper centers. In mitochondrial Complex IV, the 2 hemes are a and a3 and the 2 coppers are CuA and CuB. The 2 hemes and CuB are bound to subunit I. Acin-Perez et al.(2003) identified a cell line containing single and double missense mutations in the cytochrome c oxidase(COX) subunit I gene of mouse mitochondrial DNA. And they hypothesized that deleterious mutations can arise and become predominant; cultured cells can maintain several mtDNA haplotypes at stable frequencies; the respiratory chain has little spare COX capacity; and that the size of a cavity in the vicinity of val421 in MTCO1I of animal COX may affect the function of the enzyme.

NCBI and Uniprot Product Information

• NCBI GI #: 116982
• UniProt Accession #: P00397
• NCBI Official Full Name: Cytochrome c oxidase subunit 1
• UniProt Protein Name: Cytochrome c oxidase subunit 1
• UniProt Gene Name: Mtco1
• UniProt Synonym Gene Names: COI; mt-Co1
• UniProt Entry Name: COX1_MOUSE