IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using MTCO1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Rabbit MTCO1 Polyclonal Antibody | anti-MTCO1 antibody
MTCO1 Rabbit pAb
IHC: 1:50-1:100
IF: 1:50-1:100
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using MTCO1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of PC-12 cells using MTCO1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using MTCO1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cells using MTCO1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse lung using MTCO1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse lung using MTCO1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human stomach using MTCO1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human stomach using MTCO1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat kidney using MTCO1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat kidney using MTCO1 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using MTCO1 antibody at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using MTCO1 antibody at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
NCBI and Uniprot Product Information
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Product Notes
The MTCO1 mt-co1 (Catalog #AAA28355) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MTCO1 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MTCO1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence). WB: 1:500-1:2000 IHC: 1:50-1:100 IF: 1:50-1:100. Researchers should empirically determine the suitability of the MTCO1 mt-co1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MTCO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What does MTCO1 stand for and what is its role in mitochondria?
MTCO1 stands for Mitochondrially Encoded Cytochrome c Oxidase Subunit I. It is an essential protein in mitochondria that helps cells produce energy. It is part of the respiratory chain that converts oxygen and nutrients into usable cellular energy.
Is MTCO1 a reliable marker for mitochondrial content or activity?
Yes, MTCO1 is a reliable marker for mitochondrial content or activity. This is only found in mitochondria and is involved in energy production. It shows mitochondrial amount and hints at activity or overall cell health.
How does MTCO1 differ from other mitochondrial markers like MTCO2 or TOM20?
MTCO1 and MTCO2 are both inner mitochondrial membrane proteins involved in energy production. Whereas TOM20 is different because it's present on the outer membrane and helps import proteins.
Can the MTCO1 antibody detect protein in both human and mouse samples?
Yes, the MTCO1 antibody can detect protein in both human and mouse samples. This makes it useful to study mitochondria in patient tissue and in lab animal models.
Can this antibody be used for immunohistochemistry or flow cytometry staining?
Yes, this antibody can be used for immunohistochemistry or flow cytometry staining. This helps visualize MTCO1 in tissues and cells.
What are the recommended applications and working dilutions for MTCO1 Rabbit pAb?
The MTCO1 Rabbit polyclonal antibody is recommended for Western blot, immunohistochemistry, immunofluorescence, and ELISA. Working dilutions depend on the experiment and sample type, so users usually follow the datasheet and optimize conditions in their lab.
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