IF (Immunofluorescence)
(Confocal immunofluorescent analysis of mTOR (FRAP1) Antibody (S2481) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
Rabbit mTOR (FRAP1) Polyclonal Antibody | anti-MTOR antibody
mTOR (FRAP1) Antibody (S2481)
Gene Names
MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Immunofluorescence, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
mTOR (FRAP1), Antibody; mTOR (FRAP1) Antibody (S2481); Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; mTOR; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1; MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; anti-MTOR antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This mTOR (FRAP1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2459-2488 amino acids from human mTOR (FRAP1).
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.35 (varies by lot)
Sequence Positions
2459-2488
Sequence Length
2549
Applicable Applications for anti-MTOR antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of mTOR (FRAP1) Antibody (S2481) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of mTOR (FRAP1) Antibody (S2481) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with FRAP1-pS2481, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with FRAP1-pS2481, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of FRAP1 (arrow) using rabbit polyclonal FRAP1 Antibody (S2481). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FRAP1 gene (Lane 2) (Origene Technologies).)
WB (Western Blot)
(Western blot analysis of FRAP1 (arrow) using rabbit polyclonal FRAP1 Antibody (S2481). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FRAP1 gene (Lane 2) (Origene Technologies).)
Related Product Information for anti-MTOR antibody
FRAP1 belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. FRAP1 is a part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.
Product Categories/Family for anti-MTOR antibody
References
Dowling,R.J., Cancer Res. 67 (22), 10804-10812 (2007)
Bai,X., Science 318 (5852), 977-980 (2007)
Zhou,J., Proc. Natl. Acad. Sci. U.S.A. 104 (41), 16158-16163 (2007)
Radulovic,S., J BUON 12 SUPPL 1, S151-S162 (2007)
Bai,X., Science 318 (5852), 977-980 (2007)
Zhou,J., Proc. Natl. Acad. Sci. U.S.A. 104 (41), 16158-16163 (2007)
Radulovic,S., J BUON 12 SUPPL 1, S151-S162 (2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
288892
NCBI Official Full Name
serine/threonine-protein kinase mTOR
NCBI Official Synonym Full Names
mechanistic target of rapamycin (serine/threonine kinase)
NCBI Official Symbol
MTOR
NCBI Official Synonym Symbols
FRAP; FRAP1; FRAP2; RAFT1; RAPT1
NCBI Protein Information
serine/threonine-protein kinase mTOR
UniProt Protein Name
Serine/threonine-protein kinase mTOR
UniProt Gene Name
MTOR
UniProt Synonym Gene Names
FRAP; FRAP1; FRAP2; RAFT1; RAPT1; mTOR
UniProt Entry Name
MTOR_HUMAN
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Product Notes
The MTOR mtor (Catalog #AAA286488) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 2459-2488. The mTOR (FRAP1) Antibody (S2481) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's mTOR (FRAP1) can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MTOR mtor for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "mTOR (FRAP1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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