Application Data
(Anti-MUL1 Antibody (C-term) at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit MUL1 Polyclonal Antibody | anti-MUL1 antibody
MUL1 Antibody (C-term)
Gene Names
MUL1; GIDE; MAPL; MULAN; RNF218; C1orf166
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MUL1, Antibody; MUL1 Antibody (C-term); Mitochondrial ubiquitin ligase activator of NFKB 1; 632-; E3 SUMO-protein ligase MUL1; E3 ubiquitin-protein ligase MUL1; Growth inhibition and death E3 ligase; Mitochondrial-anchored protein ligase; MAPL; Putative NF-kappa-B-activating protein 266; RING finger protein 218; MUL1; C1orf166; GIDE; MULAN; RNF218; anti-MUL1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Monkey)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MUL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-301 amino acids from the C-terminal region of human MUL1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
272-301
Applicable Applications for anti-MUL1 antibody
ELISA, WB (Western Blot)
Function
Exhibits weak E3 ubiquitin-protein ligase activity (PubMed: , PubMed:19407830,PubMed: ). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates (PubMed:18591963,PubMed:19407830, PubMed:22410793). Can ubiquitinate AKT1 preferentially at 'Lys-284'involving 'Lys-48'-linked polyubiquitination and seems to be involved in regulation of Akt signaling by targeting phosphorylated Akt to proteosomal degradation (PubMed: ). Proposed to preferentially act as a SUMO E3 ligase at physiological concentrations (PubMed: ). Plays a role in the control of mitochondrial morphology by promoting mitochondrial fragmentation, and influences mitochondrial
localization (PubMed: , PubMed:18207745,PubMed: ). Likely to promote mitochondrial fission through negatively regulating the mitochondrial fusion proteins MFN1 and MFN2, acting in a pathway that is parallel to the PRKN/PINK1 regulatory pathway (PubMed: ). May also be involved in the sumoylation of the membrane fission protein DNM1L (PubMed: , PubMed:19407830).Inhibits cell growth (PubMed: , PubMed:22410793). When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase dependent apoptosis (PubMed: ). Involved in the modulation of innate immune defense against viruses by inhibiting DDX58-dependent antiviral response (PubMed: ). Can mediate DDX58 sumoylation and disrupt its polyubiquitination (PubMed: ).
Cellular Location
Mitochondrion outer membrane; Multi-pass membrane protein. Peroxisome. Note=Transported in mitochondrion-derived vesicles from the mitochondrion to the peroxisome.
Tissue Location
Widely expressed with highest levels in the heart, skeletal muscle, placenta, kidney and liver. Barely detectable in colon and thymus.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Application Data
(Anti-MUL1 Antibody (C-term) at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Application Data
(Anti-MUL1 Antibody (C-term) at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-MUL1 antibody
E3 ubiquitin-protein ligase that plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Product Categories/Family for anti-MUL1 antibody
References
Laure, L., et al. FEBS J. 277(20):4322-4337(2010)
Braschi, E., et al. EMBO Rep. 10(7):748-754(2009)
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Zhang, B., et al. Cell Res. 18(9):900-910(2008)
Zhang, H., et al. Biochem. Biophys. Res. Commun. 366(4):898-904(2008)
Braschi, E., et al. EMBO Rep. 10(7):748-754(2009)
Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
Zhang, B., et al. Cell Res. 18(9):900-910(2008)
Zhang, H., et al. Biochem. Biophys. Res. Commun. 366(4):898-904(2008)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39800
NCBI Official Full Name
mitochondrial ubiquitin ligase activator of NFKB 1
NCBI Official Synonym Full Names
mitochondrial E3 ubiquitin protein ligase 1
NCBI Official Symbol
MUL1
NCBI Official Synonym Symbols
GIDE; MAPL; MULAN; RNF218; C1orf166
NCBI Protein Information
mitochondrial ubiquitin ligase activator of NFKB 1
UniProt Protein Name
Mitochondrial ubiquitin ligase activator of NFKB 1
UniProt Gene Name
MUL1
UniProt Synonym Gene Names
C1orf166; GIDE; MAPL; MULAN; RNF218; MAPL
UniProt Entry Name
MUL1_HUMAN
Similar Products
Product Notes
The MUL1 mul1 (Catalog #AAA287068) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 272-301. The MUL1 Antibody (C-term) reacts with Human (Predicted Reactivity: Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's MUL1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MUL1 mul1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MUL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.