Rabbit MUSK Polyclonal Antibody | anti-MUSK antibody

MUSK Antibody (N-term)

Cat. Num. AAA285323

• Gene Names: MUSK; CMS9
• Reactivity: Human (Predicted Reactivity: Mouse, Rat)
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: MUSK, Antibody; MUSK Antibody (N-term); Muscle; skeletal receptor tyrosine-protein kinase; Muscle-specific tyrosine-protein kinase receptor; MuSK; Muscle-specific kinase receptor; MUSK; anti-MUSK antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This MUSK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-65 amino acids from the N-terminal region of human MUSK.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Sequence Positions: 35-65
• Sequence Length: 773

• Applicable Applications for anti-MUSK antibody: IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
• Function: Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle (PubMed:25537362). Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation (By similarity).
• Cellular Location: Postsynaptic cell membrane; Single-pass type I membrane protein. Note=Colocalizes with acetylcholine receptors (AChR) to the postsynaptic cell membrane of the neuromuscular junction.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemistry)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot)

(HA-tagged MuSK and GFP constructs were transfected into C2C12 cells. Transfected C2C12 cells were treated with cycloheximide (CHX) and at different times after addition of CHX, amounts of WT and mutant MuSK were analyzed by WB with anti-HA antibody. Alpha-tubulin was used as an internal control and transfection efficiency was verified with anti-GFP antibody.)

WB (Western Blot)

(MuSK protein expression in extracts of COS cells after transfection with MuSK mutated and GFP constructs. WB with polyclonal MuSK and monoclonal GFP antibodies showed normal expression of the wild-type MuSK protein (WT), diminished expression of the GA mutant MuSK and no expression of the insC mutant or the pcDNA3 vector alone in transfected COS cells. GFP cotransfection was used to verify transfection efficiency.)

FCM/FACS (Flow Cytometry)

(MUSK Antibody (N-term) (Cat. # AAA285323) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(Western blot analysis of MUSK Antibody (N-term) (Cat. # AAA285323) in Jurkat cell line lysates (35ug/lane). MUSK (arrow) was detected using the purified Pab.)

Related Product Information for anti-MUSK antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Product Categories/Family for anti-MUSK antibody

Signal Transduction

References

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Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359.
Robertson, S. et al. Trends Genet. 2000. 16: 368.
Robinson D, et al. Oncogene 2000. 19: 5548.
Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889.
Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561.
Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.

NCBI and Uniprot Product Information

• NCBI GI #: 261878455
• NCBI GeneID: 4593
• NCBI Accession #: NP_001159752.1; NP_001159753.1; NP_005583.1
• NCBI GenBank Nucleotide #: NM_001166280.1
• UniProt Accession #: O15146; Q32MJ8; Q32MJ9; Q5VZW7; Q5VZW8
• NCBI Official Full Name: muscle, skeletal receptor tyrosine-protein kinase isoform 2
• NCBI Official Synonym Full Names: muscle, skeletal, receptor tyrosine kinase
• NCBI Official Symbol: MUSK
• NCBI Official Synonym Symbols: CMS9
• NCBI Protein Information: muscle, skeletal receptor tyrosine-protein kinase
• UniProt Protein Name: Muscle, skeletal receptor tyrosine-protein kinase
• UniProt Gene Name: MUSK
• UniProt Synonym Gene Names: MuSK; Muscle-specific kinase receptor
• UniProt Entry Name: MUSK_HUMAN