IP (Immunoprecipitation)
(MVP was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate4 uL anti-MVP rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MVP rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 110 kDaObserved band size: 110 kDa)
Rabbit anti-Human MVP Polyclonal Antibody | anti-MVP antibody
Anti-MVP Antibody, Rabbit Polyclonal
Gene Names
MVP; LRP; VAULT1
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
MVP, Antibody; Anti-MVP Antibody, Rabbit Polyclonal; MVP/major vault protein Antibody, Rabbit PAb, Antigen Affinity Purified; major vault protein; Anti-LRP Antibody; Anti-VAULT1 Antibody; anti-MVP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human MVP/major vault protein
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-MVP antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human MVP/major vault protein
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human MVP/major vault protein, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(MVP was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate4 uL anti-MVP rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MVP rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 110 kDaObserved band size: 110 kDa)
IP (Immunoprecipitation)
(MVP was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate4 uL anti-MVP rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MVP rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 110 kDaObserved band size: 110 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of MVP in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human MVP polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of MVP in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human MVP polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)
WB (Western Blot)
(Anti-MVP rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:110 kDaObserved band size:120 kDa)
WB (Western Blot)
(Anti-MVP rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:110 kDaObserved band size:120 kDa)
Related Product Information for anti-MVP antibody
MVP (Major Vault Protein) is a Protein Coding gene. This gene encodes the major component of the vault complex. The encoded protein may play a role in multiple cellular processes by regulating the MAP kinase, JAK/STAT, and phosphoinositide 3-kinase/Akt signaling pathways. MVP is a ribonucleoprotein comprising a major part (>70%) of the vault particle. The human MVP is also known as lung resistance-related protein (LRP) represents the predominant component of vaults that may be involved in the defense against xenobiotics. The human MVP has been implicated in the development of drug resistance in cancer cells. MVP has been implicated in multidrug resistance, cellular transport, and malignant transformation. Overexpression of MVP has also been reported in brain tissue samples from the antiepileptic drug (AED)-resistant human focal epilepsies.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
99,327 Da
NCBI Official Full Name
major vault protein
NCBI Official Synonym Full Names
major vault protein
NCBI Official Symbol
MVP
NCBI Official Synonym Symbols
LRP; VAULT1
NCBI Protein Information
major vault protein; lung resistance-related protein
UniProt Protein Name
Major vault protein
UniProt Gene Name
MVP
UniProt Synonym Gene Names
LRP; MVP
UniProt Entry Name
MVP_HUMAN
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Product Notes
The MVP mvp (Catalog #AAA254717) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MVP Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MVP can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MVP mvp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MVP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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