Goat MYD88 Polyclonal Antibody | anti-MYD88 antibody

Goat anti-MYD88 Antibody

Cat. Num. AAA13652

• Gene Names: MYD88; MYD88D
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: MYD88, Antibody; Goat anti-MYD88 Antibody; Goat Anti-MYD88, Biotinylated Antibody; MYD88; myeloid differentiation primary response gene (88); MYD88D; myeloid differentiation primary response gene 88; MYD88 antibody; myeloid differentiation primary response gene (88) antibody; MYD88D antibody; myeloid differentiation primary response gene 88 antibody; anti-MYD88 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: IKYKAMKKEFP
• Sequence Length: 317

• Applicable Applications for anti-MYD88 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC)
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:128000.; ; Western blot: Approx 28kDa band observed in Human Spleen lysates (calculated MW of 28.3kDa according to NP_001166039.2). Recommended concentration: 0.3-0.5?g/ml. Primary incubation1 hour at room temperature.; ; IHC: Paraffin embedded Human Tonsil. Recommended concentration: 4-6?g/ml.; ; Immunofluorescence: Strong expression of the protein seen in U2OS and Jurkat cells. Recommended concentration: 10?g/ml.; ; Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
• Immunogen: Peptide with sequence C-IKYKAMKKEFP, from the internal region of the protein sequence according to NP_001166038.1; NP_002459.2; NP_001166039.1.
• Note: This antibody is expected to recognize reported isoforms 1, 2, 3 and 9
• Preparation and Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

FCM (Flow Cytometry)

(Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody)

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

IF (Immunofluorescence)

(Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml))

Application Data

(Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.)

Application Data

((6ug/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)

Application Data

((AAA13652) (0.5µg/ml) staining of Human Spleen lysate (35µg protein in RIPA buffer). Detected by chemiluminescence)

Application Data

((5ug/ml) as the reporter with as the capture rabbit antibody (5ug/ml).)

IHC (Immunohistochemistry)

((4ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not been obtained after antigen retireval at pH6 with this batch of antibody.)

WB (Western Blot)

(Staining (0.2ug/ml) of human thymus lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 289546503
• NCBI GeneID: 4615
• NCBI Accession #: NP_001166038.1; NP_002459.2; NP_001166039.1
• NCBI GenBank Nucleotide #: NM_001172567.1
• NCBI Official Full Name: myeloid differentiation primary response protein MyD88 isoform 1
• NCBI Official Synonym Full Names: myeloid differentiation primary response 88
• NCBI Official Symbol: MYD88
• NCBI Official Synonym Symbols: MYD88D
• NCBI Protein Information: myeloid differentiation primary response protein MyD88
• UniProt Protein Name: Myeloid differentiation primary response protein MyD88
• UniProt Gene Name: MYD88
• UniProt Entry Name: MYD88_HUMAN