WB (Western Blot)
(Western blot analysis of lysate from A431 cell line, using MYH14 Antibody (Center). AAA287059 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Rabbit MYH14 Polyclonal Antibody | anti-MYH14 antibody
MYH14 Antibody (Center)
Gene Names
MYH14; DFNA4; MHC16; MYH17; PNMHH; DFNA4A; myosin; FP17425; NMHC II-C; NMHC-II-C
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MYH14, Antibody; MYH14 Antibody (Center); Myosin-14; Myosin heavy chain 14; Myosin heavy chain; non-muscle IIc; Non-muscle myosin heavy chain IIc; NMHC II-C; MYH14; KIAA2034; anti-MYH14 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MYH14 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 654-668 amino acids from the Central region of human MYH14.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
2,003
Applicable Applications for anti-MYH14 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Cellular myosin that appears to play a role incytokinesis, cell shape, and specializedfunctions such as secretion and capping.
Tissue Location
High levels of expression are found in brain(highest in corpus callosum), heart, kidney,liver, lung, small intestine, colon and skeletalmuscle. Expression is low in organscomposed mainly of smooth muscle, such asaorta, uterus and urinary bladder. Nodetectable expression is found in thymus,spleen, placenta and lymphocytes.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysate from A431 cell line, using MYH14 Antibody (Center). AAA287059 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
WB (Western Blot)
(Western blot analysis of lysate from A431 cell line, using MYH14 Antibody (Center). AAA287059 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H.brain section using MYH14 Antibody (Center). AAA287059 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H.brain section using MYH14 Antibody (Center). AAA287059 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded M.brain section using MYH14 Antibody (Center). AAA287059 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded M.brain section using MYH14 Antibody (Center). AAA287059 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Related Product Information for anti-MYH14 antibody
Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping (By similarity).
Product Categories/Family for anti-MYH14 antibody
References
Leal A.,et al.Gene 312:165-171(2003).
Nagase T.,et al.Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
Grimwood J.,et al.Nature 428:529-535(2004).
Bienvenut W.V.,et al.Submitted (NOV-2006) to UniProtKB.
Jana S.S.,et al.J. Biol. Chem. 284:11563-11571(2009).
Nagase T.,et al.Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
Grimwood J.,et al.Nature 428:529-535(2004).
Bienvenut W.V.,et al.Submitted (NOV-2006) to UniProtKB.
Jana S.S.,et al.J. Biol. Chem. 284:11563-11571(2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
227871
NCBI Official Full Name
myosin-14 isoform 1
NCBI Official Synonym Full Names
myosin, heavy chain 14, non-muscle
NCBI Official Symbol
MYH14
NCBI Official Synonym Symbols
DFNA4; MHC16; MYH17; PNMHH; DFNA4A; myosin; FP17425; NMHC II-C; NMHC-II-C
NCBI Protein Information
myosin-14
UniProt Protein Name
Myosin-14
UniProt Gene Name
MYH14
UniProt Synonym Gene Names
KIAA2034; NMHC II-C
UniProt Entry Name
MYH14_HUMAN
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Product Notes
The MYH14 myh14 (Catalog #AAA287059) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYH14 Antibody (Center) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's MYH14 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MYH14 myh14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYH14, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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