Rabbit MYL2 Polyclonal Antibody | anti-MYL2 antibody

MYL2 Antibody (Center)

Cat. Num. AAA290890

• Gene Names: MYL2; MLC2; CMH10; MLC-2s/v
• Reactivity: Human, Mouse, Rat; Predicted: Chicken, Rabbit
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified through a protein A column, followed by peptide affinity purification.
• Synonyms: MYL2, Antibody; MYL2 Antibody (Center); Purified Rabbit Polyclonal Antibody (Pab); Myosin regulatory light chain 2; ventricular/cardiac muscle isoform; MLC-2; MLC-2v; MYL2; anti-MYL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Chicken, Rabbit
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This MYL2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 42-75 amino acids from the Central region of human MYL2.
• Purity/Purification: Purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 166

• Applicable Applications for anti-MYL2 antibody: ELISA, WB (Western Blot), FCM/FACS (Flow Cytometry)
• Antigen Type: Synthesized Peptide
• Antigen Source: Human
• Cellular Location: Cytoplasm, myofibril, sarcomere, A band {ECO:0000250|UniProtKB:P08733}
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks.; For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(All lanes : Anti-MYL2 Antibody (Center) at 1:2000 dilutionLane 1: Human heart lysateLane 2: Human skeletal muslce lysateLane 3: Mouse heart lysateLane 4: Rat heart lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 19 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

FCM/FACS (Flow Cytometry)

(Overlay histogram showing U-2 OS cells stained with AP22304c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22304c, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

Related Product Information for anti-MYL2 antibody

Contractile protein that plays a role in heart development and function (By similarity). Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force (By similarity). During cardiogenesis plays an early role in cardiac contractility by promoting cardiac myofibril assembly (By similarity).

Product Categories/Family for anti-MYL2 antibody

Primary Antibodies; Cardiovascular; Signal Transduction; Stem Cells

References

Libera L.D.,et al.Nucleic Acids Res. 17:2360-2360(1989). Wu Q.L.,et al. Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases. Margossian S.S.,et al. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases. Wadgaonkar R.,et al.Cell. Mol. Biol. Res. 39:13-26(1993). Kovalyov L.I.,et al.Electrophoresis 16:1160-1169(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 94981553
• NCBI GeneID: 4633
• NCBI Accession #: NP_000423.2
• NCBI GenBank Nucleotide #: NM_000432.3
• UniProt Accession #: P10916; Q16123
• Molecular Weight: 18789
• NCBI Official Full Name: myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• NCBI Official Synonym Full Names: myosin light chain 2
• NCBI Official Symbol: MYL2
• NCBI Official Synonym Symbols: MLC2; CMH10; MLC-2s/v
• NCBI Protein Information: myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• UniProt Protein Name: Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• UniProt Gene Name: MYL2
• UniProt Synonym Gene Names: MLC-2s/v