Rabbit anti-Human MYLK Polyclonal Antibody | anti-MYLK antibody

MYLK Antibody

Cat. Num. AAA233356

• Gene Names: MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
• Reactivity: Human
• Applications: Immunofluorescence, Immunohistochemistry, ELISA
• Purity: >95%, Protein G purified
• Synonyms: MYLK, Antibody; MYLK Antibody; Myosin light chain kinase, smooth muscle; MLCK; smMLCK; Kinase-related protein; KRP; Telokin; Myosin light chain kinase; smooth muscle; deglutamylated form; MYLK; MLCK1; MYLK1; anti-MYLK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 1738

• Applicable Applications for anti-MYLK antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
• Immunogen: Recombinant Human Myosin light chain kinase, smooth muscle protein (290-398AA)
• Conjugation: Non-conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

IF (Immunofluorescence)

(Immunofluorescent analysis of HepG2 cells using AAA233356 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human smooth muscle tissue using AAA233356 at dilution of 1:100)

Related Product Information for anti-MYLK antibody

Calcium/calmodulin-dependent myosin light chain kinase implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activity. Phosphorylates PTK2B/PYK2 and myosin light-chains. Involved in the inflammatory response (e.g. apoptosis, vascular permeability, leukocyte diapedesis), cell motility and morphology, airway hyperreactivity and other activities relevant to asthma. Required for tonic airway smooth muscle contraction that is necessary for physiological and asthmatic airway resistance. Necessary for gastrointestinal motility. Implicated in the regulation of endothelial as well as vascular permeability, probably via the regulation of cytoskeletal rearrangements. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis. Plays a role in the regulation of epithelial cell survival. Required for epithelial wound healing, especially during actomyosin ring contraction during purse-string wound closure. Mediates RhoA-dependent membrane blebbing. Triggers TRPC5 channel activity in a calcium-dependent signaling, by inducing its subcellular localization at the plasma membrane. Promotes cell migration (including tumor cells) and tumor metastasis. PTK2B/PYK2 activation by phosphorylation mediates ITGB2 activation and is thus essential to trigger neutrophil transmigration during acute lung injury (ALI). May regulate optic nerve head astrocyte migration. Probably involved in mitotic cytoskeletal regulation. Regulates tight junction probably by modulating ZO-1 exchange in the perijunctional actomyosin ring. Mediates burn-induced microvascular barrier injury; triggers endothelial contraction in the development of microvascular hyperpermeability by phosphorylating MLC. Essential for intestinal barrier dysfunction. Mediates Giardia spp.-mediated reduced epithelial barrier function during giardiasis intestinal infection via reorganization of cytoskeletal F-actin and tight junctional ZO-1. Necessary for hypotonicity-induced Ca(2+) entry and subsequent activation of volume-sensitive organic osmolyte/anion channels (VSOAC) in cervical cancer cells. Responsible for high proliferative ability of breast cancer cells through anti-apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 1008806537
• NCBI GeneID: 4638
• NCBI Accession #: NP_001308238.1
• NCBI GenBank Nucleotide #: NM_001321309.1
• UniProt Accession #: Q15746; O95796; O95797; O95798; O95799; Q14844; Q16794; Q17S15; Q3ZCP9; Q5MY99; B4DUE3; D3DN97
• Molecular Weight: 110,076 Da
• NCBI Official Full Name: myosin light chain kinase, smooth muscle isoform 9
• NCBI Official Synonym Full Names: myosin light chain kinase
• NCBI Official Symbol: MYLK
• NCBI Official Synonym Symbols: KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
• NCBI Protein Information: myosin light chain kinase, smooth muscle
• UniProt Protein Name: Myosin light chain kinase, smooth muscle
• UniProt Gene Name: MYLK
• UniProt Synonym Gene Names: MLCK; MLCK1; MYLK1; MLCK; smMLCK; KRP