WB (Western Blot)
(Western blot analysis MYH4 in mouse C2C12 (lane 1) and mouse extraocular muscle (lane 2). Both lanes of the blot were probed with rabbit polyclonal anti-MYH4/MyHC-IIB (C-terminus) at 1:1000.)
Rabbit Myosin 4/MyHC-IIB Polyclonal Antibody | anti-MyHC-IIB antibody
Myosin 4/MyHC-IIB (C-terminus) Antibody
Gene Names
Myh4; MM; MHC2B; Myhsf; MYH-2B; Minmus; Minimsc; AI506973; MyHC-IIb
Applications
ELISA, Western Blot
Synonyms
Myosin 4/MyHC-IIB, Antibody; Myosin 4/MyHC-IIB (C-terminus) Antibody; Myosin 4/MyHC-IIB (C-terminus); Myosin HC04; anti-MyHC-IIB antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
This antibody was affinity purified using MYH4 (C-terminus) peptide (without carrier). The antibody detects a 215kDa* protein corresponding to the apparent molecular mass of MYH4 on SDS-PAGE immunoblots of mouse extraocular muscle and mouse C2C12 cells. The antibody may also detect other skeletal MYHs, such as MYH1 and MYH2, which have significant homology to the C-terminal region of MYH4.
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-MyHC-IIB antibody
ELISA, WB (Western Blot)
Immunogen
A synthetic peptide (coupled to KLH) corresponding to amino acid residues at the C-terminus from mouse MYH4/MyHC-IIB. This sequence has 100% identity with rat and human MYH4, and has significant homology to MYH1, MYH2, and MYH8.
Preparation and Storage
Store at -20 degree C. Do not aliquot. Stable for 1 year.
WB (Western Blot)
(Western blot analysis MYH4 in mouse C2C12 (lane 1) and mouse extraocular muscle (lane 2). Both lanes of the blot were probed with rabbit polyclonal anti-MYH4/MyHC-IIB (C-terminus) at 1:1000.)
WB (Western Blot)
(Western blot analysis MYH4 in mouse C2C12 (lane 1) and mouse extraocular muscle (lane 2). Both lanes of the blot were probed with rabbit polyclonal anti-MYH4/MyHC-IIB (C-terminus) at 1:1000.)
Related Product Information for anti-MyHC-IIB antibody
Myosin is a highly conserved, ubiquitous protein found in all eukaryotic cells, where it provides the motor function for diverse movements such as cytokinesis, phagocytosis, and muscle contraction. All myosins contain an amino-terminal motor/head domain and a carboxy-terminal tail domain. The class II myosins, consist of the conventional two-headed myosins that form filaments and are composed of two myosin heavy chain (MYH) subunits and four myosin light chain subunits. There are 15 MYH genes identified as Class II myosins and these include six skeletal muscle MYHs (MYH1, MYH2, MYH3, MYH4, MYH8, MYH13), three cardiac MYHs (MYH6, MYH7, MYH7B) and two non-muscle MYHs (MYH9, MYH10), as well as a smooth muscle MYH (MYH11).
References
Resnicow, D.I. et al. (2010) Proc Natl Acad Sci U S A. 107(3):1053.
Berg, J.S. et al. (2001) Mol Biol Cell. 12:780.
Berg, J.S. et al. (2001) Mol Biol Cell. 12:780.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
myosin-4
NCBI Official Synonym Full Names
myosin, heavy polypeptide 4, skeletal muscle
NCBI Official Symbol
Myh4
NCBI Official Synonym Symbols
MM; MHC2B; Myhsf; MYH-2B; Minmus; Minimsc; AI506973; MyHC-IIb
NCBI Protein Information
myosin-4
UniProt Protein Name
Myosin-4
UniProt Gene Name
Myh4
UniProt Synonym Gene Names
MyHC-2b
UniProt Entry Name
MYH4_MOUSE
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Product Notes
The MyHC-IIB myh4 (Catalog #AAA71665) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Myosin 4/MyHC-IIB can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MyHC-IIB myh4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myosin 4/MyHC-IIB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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