ICC (Immunocytochemistry)
(Immunocytometrical labeling of phosphorylated and unphosphorylated N-WASP in rabbit spleen fibroblasts. The cells were probed with N-WASP phospho-specific and N-WASP unphosphorylated antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were in the absence (left) or presence (right) or their respective blocking peptide.)
Rabbit N-WASP (Ser-484/Ser-485) Polyclonal Antibody | anti-WASL antibody
Anti-N-WASP (S484/S485), phospho-specific [WASP (S483/S484)]
Gene Names
WASL; NWASP; N-WASP; MGC48327; DKFZp779G0847
Reactivity
Human, Rat, Mouse
Applications
Immunocytochemistry, Western Blot, ELISA
Purity
Affinity Purified
Synonyms
N-WASP (Ser-484/Ser-485), Antibody; Anti-N-WASP (S484/S485), phospho-specific [WASP (S483/S484)]; N-WASP (Ser-484/Ser-485); phospho-specific; Neural Wiskott-Aldrich syndrome protein; WASL; anti-WASL antibody
Host
Rabbit
Reactivity
Human, Rat, Mouse
Clonality
Polyclonal
Specificity
This antibody was cross-adsorbed to unphosphorylated-N-WASP (S484/S485) peptide (without carrier) then affinity purified using phospho-N-WASP (S484/S485) peptide (without carrier). The antibody detects a 63 kDa* protein corresponding to the molecular mass of phosphorylated WASP on SDS-PAGE immunoblots of Jurkat cell lysate. In rat brain, A431, human endothelial and SKN-SH cells, this antibody detects a 65 kDa* protein corresponding to phosphorylated N-WASP.
Purity/Purification
Affinity Purified
Form/Format
Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-WASL antibody
ICC (Immunocytochemistry), WB (Western Blot), ELISA
Immunogen
Phospho-N-WASP (S484/S485) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 484 and 485 of human N-WASP. The human WASP sequence has a similar peptide sequence surrounding serine 483 and 484.
Preparation and Storage
Store at -20°C. Stable for 1 year.
ICC (Immunocytochemistry)
(Immunocytometrical labeling of phosphorylated and unphosphorylated N-WASP in rabbit spleen fibroblasts. The cells were probed with N-WASP phospho-specific and N-WASP unphosphorylated antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were in the absence (left) or presence (right) or their respective blocking peptide.)
ICC (Immunocytochemistry)
(Immunocytometrical labeling of phosphorylated and unphosphorylated N-WASP in rabbit spleen fibroblasts. The cells were probed with N-WASP phospho-specific and N-WASP unphosphorylated antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were in the absence (left) or presence (right) or their respective blocking peptide.)
Application Data
Application Data
Related Product Information for anti-WASL antibody
Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Phosphorylation regulates the activity of both proteins. Dual phosphorylation of WASP on serine 483 and 484 by casein kinases increase the affinity for the ARP2/3 complex. Thus, dual serine phosphorylation may be important for formation of actin-based structures in various cell types.
References
• Higgs, H.N. & Pollard, T.D. (2001) Annu Rev Biochem 70:649-676.
• Cory, G.O. et al. (2003) Mol Cell 11(5):1229-39.
• Cory, G.O. et al. (2003) Mol Cell 11(5):1229-39.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
neural Wiskott-Aldrich syndrome protein
NCBI Official Synonym Full Names
Wiskott-Aldrich syndrome-like
NCBI Official Symbol
WASL
NCBI Official Synonym Symbols
NWASP; N-WASP; MGC48327; DKFZp779G0847
NCBI Protein Information
neural Wiskott-Aldrich syndrome protein; OTTHUMP00000211994; Wiskott-Aldrich syndrome gene-like
UniProt Protein Name
Neural Wiskott-Aldrich syndrome protein
UniProt Gene Name
WASL
UniProt Entry Name
WASL_HUMAN
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Product Notes
The WASL wasl (Catalog #AAA71525) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-N-WASP (S484/S485), phospho-specific [WASP (S483/S484)] reacts with Human, Rat, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's N-WASP (Ser-484/Ser-485) can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the WASL wasl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N-WASP (Ser-484/Ser-485), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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