Rabbit N4BP1 Polyclonal Antibody | anti-N4BP1 antibody

N4BP1 Antibody

Cat. Num. AAA39777

• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
• Purity: N4BP1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: N4BP1, Antibody; N4BP1 Antibody; N4BP1; KIAA0615; NEDD4-binding protein 1; NEDD4 binding protein 1; anti-N4BP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform.
• Purity/Purification: N4BP1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 896

• Applicable Applications for anti-N4BP1 antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
• Conjugate: Unconjugated
• Immunogen: N4BP1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1.
• Buffer: N4BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: N4BP1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

IF (Immunofluorescence)

(Immunofluorescence of N4BP1 in HeLa cells with N4BP1 antibody at 20 μg/mL.)

ICC (Immunocytochemistry)

(Immunocytochemistry of N4BP1 in HeLa cells with N4BP1 antibody at 10 μg/mL.)

WB (Western Blot)

(Western blot analysis of N4BP1 in HeLa cell lysate with N4BP1 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

Related Product Information for anti-N4BP1 antibody

N4BP1 Antibody: Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.

NCBI and Uniprot Product Information

• NCBI GI #: 48928019
• NCBI GeneID: 9683
• NCBI Accession #: NP_694574
• NCBI GenBank Nucleotide #: NM_153029.3
• UniProt Accession #: O75113; Q2YDX1; A7MD49
• Molecular Weight: 100,379 Da
• NCBI Official Full Name: NEDD4-binding protein 1
• NCBI Official Synonym Full Names: NEDD4 binding protein 1
• NCBI Official Symbol: N4BP1
• NCBI Protein Information: NEDD4-binding protein 1
• UniProt Protein Name: NEDD4-binding protein 1
• UniProt Gene Name: N4BP1
• UniProt Synonym Gene Names: KIAA0615; N4BP1
• UniProt Entry Name: N4BP1_HUMAN