WB (Western Blot)
(Detection of human N4BP1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-N4BP1 antibody AAA212821 (lot AAA212821-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human N4BP1 Polyclonal Antibody | anti-N4BP1 antibody
Rabbit anti-N4BP1 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
N4BP1, Antibody; Rabbit anti-N4BP1 Antibody, Affinity Purified; N4BP1; NEDD4-binding protein 1; NEDD4 binding protein 1; anti-N4BP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-N4BP1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 250 and 300
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human N4BP1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-N4BP1 antibody AAA212821 (lot AAA212821-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human N4BP1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-N4BP1 antibody AAA212821 (lot AAA212821-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human N4BP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-N4BP1 antibody AAA212821 (lot AAA212821-1) used for IP at 6 ug per reaction. N4BP1 was also immunoprecipitated by rabbit anti-N4BP1 antibody BL17672. For blotting immunoprecipitated N4BP1, AAA212821 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human N4BP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-N4BP1 antibody AAA212821 (lot AAA212821-1) used for IP at 6 ug per reaction. N4BP1 was also immunoprecipitated by rabbit anti-N4BP1 antibody BL17672. For blotting immunoprecipitated N4BP1, AAA212821 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-N4BP1 antibody
N4BP1 is an inhibitor of the E3 ubiquitin-protein ligase ITCH. N4BP1 ccts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O75113].
Product Categories/Family for anti-N4BP1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
100,379 Da
NCBI Official Full Name
NEDD4-binding protein 1
NCBI Official Synonym Full Names
NEDD4 binding protein 1
NCBI Official Symbol
N4BP1
NCBI Protein Information
NEDD4-binding protein 1
UniProt Protein Name
NEDD4-binding protein 1
UniProt Gene Name
N4BP1
UniProt Synonym Gene Names
KIAA0615; N4BP1
UniProt Entry Name
N4BP1_HUMAN
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Product Notes
The N4BP1 n4bp1 (Catalog #AAA212821) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-N4BP1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's N4BP1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the N4BP1 n4bp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N4BP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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