Application Data
(Anti-SIRT1 Picoband antibody, AAA46089-2.jpgAll lanes: Anti SIRT1 (AAA46089) at 0.5ug/mlLane 1: HEPG2 Whole Cell Lysate at 40ugLane 2: MCF Whole Cell Lysate at 40ugLane 3: SW620 Cell Lysate at 40ugPredicted bind size: 120KDObserved bind size: 120KD)
Rabbit NAD-dependent protein deacetylase sirtuin-1 Polyclonal Antibody | anti-SIRT1 antibody
Anti-SIRT1 Antibody
Gene Names
SIRT1; SIR2L1
Reactivity
Human. No cross reactivity with other proteins.
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
NAD-dependent protein deacetylase sirtuin-1, Antibody; Anti-SIRT1 Antibody; 75SirT1 antibody; BA57G10.4 antibody; hSIR2 antibody; hSIRT1 antibody; HST2, S. cerevisiae, homolog of antibody; NAD dependent deacetylase SIRT1 antibody; NAD dependent deacetylase sirtuin 1 antibody; NAD dependent protein deacetylase sirtuin 1 antibody; OTTHUMP00000198111 antibody; OTTHUMP00000198112 antibody; Regulatory protein SIR2 homolog 1 antibody; SIR1_HUMAN antibody; SIR2 like 1 antibody; SIR2 like protein 1 antibody; SIR2, S.cerevisiae, homolog-like 1 antibody; SIR2-like protein 1 antibody; SIR2ALPHA antibody; SIR2alpha protein antibody; SIR2L1 antibody; SIRT 1 antibody; Sirt1 antibody; SIRT1 Sir2 like proteins (siruitins) type 1 antibody; SIRT1: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody; SirtT1 75 kDa fragment antibody; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody; Sirtuin 1 antibody; sirtuin antibody; Sirtuin type 1 antibody; sirtuin 1; anti-SIRT1 antibody
Host
Rabbit
Reactivity
Human. No cross reactivity with other proteins.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
452
Applicable Applications for anti-SIRT1 antibody
WB (Western Blot)
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E.coli-derived human SIRT1 recombinant protein (Position: R112-K311). Human SIRT1 shares 90% amino acid (aa) sequence identity with mouse SIRT1.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Application Data
(Anti-SIRT1 Picoband antibody, AAA46089-2.jpgAll lanes: Anti SIRT1 (AAA46089) at 0.5ug/mlLane 1: HEPG2 Whole Cell Lysate at 40ugLane 2: MCF Whole Cell Lysate at 40ugLane 3: SW620 Cell Lysate at 40ugPredicted bind size: 120KDObserved bind size: 120KD)
Application Data
(Anti-SIRT1 Picoband antibody, AAA46089-2.jpgAll lanes: Anti SIRT1 (AAA46089) at 0.5ug/mlLane 1: HEPG2 Whole Cell Lysate at 40ugLane 2: MCF Whole Cell Lysate at 40ugLane 3: SW620 Cell Lysate at 40ugPredicted bind size: 120KDObserved bind size: 120KD)
Application Data
(Anti-SIRT1 Picoband antibody, AAA46089-1.jpgAll lanes: Anti SIRT1 (AAA46089) at 0.5ug/mlWB: Recombinant Human SIRT1 Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
Application Data
(Anti-SIRT1 Picoband antibody, AAA46089-1.jpgAll lanes: Anti SIRT1 (AAA46089) at 0.5ug/mlWB: Recombinant Human SIRT1 Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
Related Product Information for anti-SIRT1 antibody
Description: Rabbit IgG polyclonal antibody for NAD-dependent protein deacetylase sirtuin-1(SIRT1) detection. Tested with WB in Human.
Background: Sirtuin 1, also known as SIR2L1 or SIRT1, is a protein that in humans is encoded by the SIRT1 gene. It is mapped to 10q21.3. Sirtuin 1 is a member of the sirtuin family of proteins, homologs of the Sir2 gene in S. cerevisiae. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. Sirtuin 1 is downregulated in cells that have high insulin resistance and inducing its expression increases insulin sensitivity, suggesting the molecule is associated with improving insulin sensitivity. Furthermore, Sirtuin 1 was shown to de-acetylate and affect the activity of both members of the PGC1-alpha/ERR-alpha complex, which are essential metabolic regulatory transcription factors.
Background: Sirtuin 1, also known as SIR2L1 or SIRT1, is a protein that in humans is encoded by the SIRT1 gene. It is mapped to 10q21.3. Sirtuin 1 is a member of the sirtuin family of proteins, homologs of the Sir2 gene in S. cerevisiae. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. Sirtuin 1 is downregulated in cells that have high insulin resistance and inducing its expression increases insulin sensitivity, suggesting the molecule is associated with improving insulin sensitivity. Furthermore, Sirtuin 1 was shown to de-acetylate and affect the activity of both members of the PGC1-alpha/ERR-alpha complex, which are essential metabolic regulatory transcription factors.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,066 Da
NCBI Official Full Name
NAD-dependent protein deacetylase sirtuin-1 isoform b
NCBI Official Synonym Full Names
sirtuin 1
NCBI Official Symbol
SIRT1
NCBI Official Synonym Symbols
SIR2L1
NCBI Protein Information
NAD-dependent protein deacetylase sirtuin-1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; SIR2-like protein 1; regulatory protein SIR2 homolog 1; NAD-dependent deacetylase sirtuin-1
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-1
UniProt Gene Name
SIRT1
UniProt Synonym Gene Names
SIR2L1; hSIRT1; hSIR2; 75SirT1
UniProt Entry Name
SIR1_HUMAN
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Product Notes
The SIRT1 sirt1 (Catalog #AAA46089) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SIRT1 Antibody reacts with Human. No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's NAD-dependent protein deacetylase sirtuin-1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the SIRT1 sirt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAD-dependent protein deacetylase sirtuin-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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