WB (Western Blot)
(Detection of human NAF1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-NAF1 antibody AAA212368 (lot AAA212368-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human NAF1 Polyclonal Antibody | anti-NAF1 antibody
Rabbit anti-NAF1 Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NAF1, Antibody; Rabbit anti-NAF1 Antibody, Affinity Purified; H/ACA ribonucleoprotein complex non-core subunit NAF1; hNAF1; nuclear assembly factor 1 homolog; nuclear assembly factor 1 ribonucleoprotein; anti-NAF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-NAF1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 50 and 100
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human NAF1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-NAF1 antibody AAA212368 (lot AAA212368-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human NAF1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 ug), HeLa (50ug), and Jurkat (50ug) cells. Antibodies: Affinity purified rabbit anti-NAF1 antibody AAA212368 (lot AAA212368-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human NAF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-NAF1 antibody AAA212368 (lot AAA212368-1) used for IP at 6 ug/mg lysate. NAF1 was also immunoprecipitated by rabbit anti-NAF1 antibody BL14075. For blotting immunoprecipitated NAF1, AAA212368 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human NAF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-NAF1 antibody AAA212368 (lot AAA212368-1) used for IP at 6 ug/mg lysate. NAF1 was also immunoprecipitated by rabbit anti-NAF1 antibody BL14075. For blotting immunoprecipitated NAF1, AAA212368 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-NAF1 antibody
H/ACA ribonucleoprotein complex non-core subunit NAF1 (nuclear assembly factor 1) is an RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disappears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. NAF1 probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4 [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96HR8].
Product Categories/Family for anti-NAF1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The NAF1 naf1 (Catalog #AAA212368) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NAF1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NAF1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NAF1 naf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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