Standard Curve (Sample)
(Fig.1. Human H/ACA ribonucleoprotein complex non-core subunit NAF1 (NAF1) Standard Curve.)
Human H/ACA Ribonucleoprotein Complex Non-core Subunit NAF1 (NAF1) ELISA Kit | NAF1 elisa kit
Human H/ACA Ribonucleoprotein Complex Non-core Subunit NAF1 (NAF1) ELISA Kit
Reactivity
Human
Synonyms
H/ACA Ribonucleoprotein Complex Non-core Subunit NAF1 (NAF1); N/A; Human H/ACA Ribonucleoprotein Complex Non-core Subunit NAF1 (NAF1) ELISA Kit; NAF1; Nuclear assembly factor 1 homolog; NAF1 elisa kit
Reactivity
Human
Specificity
Human H/ACA ribonucleoprotein complex non-core subunit NAF1 (NAF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human NAF1. No significant cross-reactivity or interference between Human NAF1 and analogues was observed.
Sequence Length
389
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human H/ACA ribonucleoprotein complex non-core subunit NAF1 (NAF1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human H/ACA ribonucleoprotein complex non-core subunit NAF1 (NAF1) Standard Curve.)
Related Product Information for NAF1 elisa kit
Description: This Human H/ACA ribonucleoprotein complex non-core subunit NAF1 (NAF1) ELISA Kit employs a two-site sandwich ELISA to quantitate NAF1 in samples. An antibody specific for NAF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: NAF1 (Nuclear Assembly Factor 1 Ribonucleoprotein) is a Protein Coding gene. GO annotations related to this gene include poly(A) RNA binding and RNA binding. RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disappears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4.
Background: NAF1 (Nuclear Assembly Factor 1 Ribonucleoprotein) is a Protein Coding gene. GO annotations related to this gene include poly(A) RNA binding and RNA binding. RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disappears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
H/ACA ribonucleoprotein complex non-core subunit NAF1 isoform b
NCBI Official Synonym Full Names
nuclear assembly factor 1 ribonucleoprotein
NCBI Official Symbol
NAF1
NCBI Protein Information
H/ACA ribonucleoprotein complex non-core subunit NAF1
UniProt Protein Name
H/ACA ribonucleoprotein complex non-core subunit NAF1
UniProt Gene Name
NAF1
UniProt Synonym Gene Names
hNAF1
UniProt Entry Name
NAF1_HUMAN
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Product Notes
The Human NAF1 naf1 (Catalog #AAA331554) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331554 ELISA Kit recognizes Human NAF1. It is sometimes possible for the material contained within the vial of "H/ACA Ribonucleoprotein Complex Non-core Subunit NAF1 (NAF1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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