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WB (Western Blot)
(Anti-NAMPT rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:54 kDaObserved band size:54 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human NAMPT Polyclonal Antibody | anti-NAMPT antibody
Anti-NAMPT Antibody, Rabbit Polyclonal
Gene Names
NAMPT; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
NAMPT, Antibody; Anti-NAMPT Antibody, Rabbit Polyclonal; PBEF/Visfatin/NAMPT Antibody, Rabbit PAb, Antigen Affinity Purified; nicotinamide phosphoribosyltransferase; Anti-1110035O14Rik Antibody; Anti-PBEF Antibody; Anti-PBEF1 Antibody; Anti-VF Antibody; Anti-VISFATIN Antibody; anti-NAMPT antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human NAMPT/PBEF
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-NAMPT antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Immunogen
Recombinant Human NAMPT/PBEF protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human NAMPT/PBEF (rh NAMPT/PBEF). NAMPT/PBEF specific IgG was purified by Human NAMPT/PBEF affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-NAMPT rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:54 kDaObserved band size:54 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-NAMPT rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:54 kDaObserved band size:54 kDa(We are unsure as to the identity of these extra bands.))
IP (Immunoprecipitation)
(NAMPT was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-NAMPT rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NAMPT rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size: 55 kDa)
IP (Immunoprecipitation)
(NAMPT was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate2 uL anti-NAMPT rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-NAMPT rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilutionDeveloped using the DAB staining technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size: 55 kDa)
IHC (Immunohiostchemistry)
(Immunochemical staining of human NAMPT in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
IHC (Immunohiostchemistry)
(Immunochemical staining of human NAMPT in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human NAMPT in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry)
(Immunochemical staining of human NAMPT in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-NAMPT antibody
Nicotinamide phosphoribosyltransferase (NAMPT), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin, is an enzyme belonging to the family of glycosyltransferases, to be specific, the pentosyltransferases. This enzyme participates in nicotinate and nicotinamide metabolism. This enzyme catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. NAMPT is also considered as an essential enzyme mediating granulocyte colony-stimulating factor (G-CSF)-triggered granulopoiesis in healthy individuals and individuals with severe congenital neutropenia. Intracellular NAMPT and NAD+ amounts in myeloid cells, as well as plasma NAMPT and NAD+ levels, were increased by G-CSF treatment of both healthy volunteers and individuals with congenital neutropenia.
References
Skokowa J, et al. (2009) NAMPT is essential for the G-CSF-induced myeloid differentiation via a NAD+-sirtuin-1-dependent pathway. Nat Med. 15(2): 151-8.Samal B, et al. (1994) Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor. Mol Cell Biol. 14 (2): 1431-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
491
NCBI Official Full Name
Nicotinamide phosphoribosyltransferase
NCBI Official Synonym Full Names
nicotinamide phosphoribosyltransferase
NCBI Official Symbol
NAMPT
NCBI Official Synonym Symbols
VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
NCBI Protein Information
nicotinamide phosphoribosyltransferase; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1
UniProt Protein Name
Nicotinamide phosphoribosyltransferase
UniProt Gene Name
NAMPT
UniProt Synonym Gene Names
PBEF; PBEF1; NAmPRTase; Nampt; Pre-B cell-enhancing factor
UniProt Entry Name
NAMPT_HUMAN
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Product Notes
The NAMPT nampt (Catalog #AAA255281) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NAMPT Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NAMPT can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NAMPT nampt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAMPT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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