IF (Immunofluorescence)
((5ug/ml) staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes.)
Goat NANOG Polyclonal Antibody | anti-NANOG antibody
Goat anti-NANOG Antibody
Reactivity
Tested: Human, Pig; Expected from sequence similarity: Human, Dog, Pig
Applications
ELISA, Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
NANOG, Antibody; Goat anti-NANOG Antibody; Goat Anti-NANOG (C Terminus) Antibody; NANOG; Nanog homeobox; HGNC: 20857; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; NANOG antibody; Nanog homeobox antibody; HGNC: 20857 antibody; homeobox transcription factor Nanog antibody; homeobox transcription factor Nanog-delta 48 antibody; anti-NANOG antibody
Host
Goat
Reactivity
Tested: Human, Pig; Expected from sequence similarity: Human, Dog, Pig
Clonality
Polyclonal
Specificity
This antibody has been successfully used in ICC on Human
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
305
Applicable Applications for anti-NANOG antibody
Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.2). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
IHC: This antibody was successfully used Cell Transplant.
IF/ICC: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green).
This antibody was successfully used in IF on Human. This antibody was successfully used in ICC.
Enzyme immunoassay:This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
Western blot: Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.2). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
IHC: This antibody was successfully used Cell Transplant.
IF/ICC: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green).
This antibody was successfully used in IF on Human. This antibody was successfully used in ICC.
Enzyme immunoassay:This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse.
Immunogen
Peptide with sequence C-QNQRMKSKRWQKNN, from the internal region of the protein sequence according to NP_079141.2.
Preparation and Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
IF (Immunofluorescence)
((5ug/ml) staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes.)
IF (Immunofluorescence)
((5ug/ml) staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes.)
WB (Western Blot)
((0. 03ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
WB (Western Blot)
((0. 03ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
homeobox protein NANOG isoform 1
NCBI Official Synonym Full Names
Nanog homeobox
NCBI Official Symbol
NANOG
NCBI Protein Information
homeobox protein NANOG
UniProt Protein Name
Homeobox protein NANOG
UniProt Gene Name
NANOG
UniProt Synonym Gene Names
hNanog
UniProt Entry Name
NANOG_HUMAN
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Product Notes
The NANOG nanog (Catalog #AAA13647) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NANOG Antibody reacts with Tested: Human, Pig; Expected from sequence similarity: Human, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's NANOG can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA (EIA). Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 38kDa band observed in Human Ovary lysates (calculated MW of 34.6kDa according to NP_079141.2). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour. IHC: This antibody was successfully used Cell Transplant. IF/ICC: Parts of a keratinocyte-derived colony of induced pluripotent stem cells are stained for Nanog (green). This antibody was successfully used in IF on Human. This antibody was successfully used in ICC. Enzyme immunoassay:This antibody does not work in PFA-fixed frozen embryos and ESC from Mouse. Researchers should empirically determine the suitability of the NANOG nanog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NANOG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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